LAMB1 Antibody
| Code | Size | Price |
|---|
| PSI-57-398-400ul | 400ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
Laminin subunit beta-1, Laminin B1 chain, Laminin-1 subunit beta, Laminin-10 subunit beta, Laminin-12 subunit beta, Laminin-2 subunit beta, Laminin-6 subunit beta, Laminin-8 subunit beta, LAMB1
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For IHC-P starting dilution is: 1:10~50
Background:
Laminins, a family of extracellular matrix glycoproteins,
are the major noncollagenous constituent of basement membranes.
They have been implicated in a wide variety of biological processes
including cell adhesion, differentiation, migration, signaling,
neurite outgrowth and metastasis. Laminins are composed of 3 non
identical chains: laminin alpha, beta and gamma (formerly A, B1,
and B2, respectively) and they form a cruciform structure
consisting of 3 short arms, each formed by a different chain, and a
long arm composed of all 3 chains. Each laminin chain is a
multidomain protein encoded by a distinct gene. Several isoforms
of each chain have been described. Different alpha, beta and gamma
chain isomers combine to give rise to different heterotrimeric
laminin isoforms which are designated by Arabic numerals in the
order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is
laminin 1. The biological functions of the different chains and
trimer molecules are largely unknown, but some of the chains have
been shown to differ with respect to their tissue distribution,
presumably reflecting diverse functions in vivo. This gene encodes
the beta chain isoform laminin, beta 1. The beta 1 chain has 7
structurally distinct domains which it shares with other beta chain
isomers. The C-terminal helical region containing domains I and II
are separated by domain alpha, domains III and V contain several
EGF-like repeats, and domains IV and VI have a globular
conformation. Laminin, beta 1 is expressed in most tissues that
produce basement membranes, and is one of the 3 chains constituting
laminin 1, the first laminin isolated from Engelbreth-Holm-Swarm
(EHS) tumor. A sequence in the beta 1 chain that is involved in
cell attachment, chemotaxis, and binding to the laminin receptor
was identified and shown to have the capacity to inhibit
metastasis.
Background References:
- Joslyn, G., et al. Alcohol. Clin. Exp. Res. 34(5):800-812(2010)
- McGovern, D.P., et al. Nat. Genet. 42(4):332-337(2010)
- Wheeler, H.E., et al. PLoS Genet.(10), E1000685 (2009) :
- Rooney, J.E., et al. Proc. Natl. Acad. Sci. U.S.A. 106(19):7991-7996(2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This LAMB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1691-1720 amino acids from the C-terminal region of human LAMB1.
NCBI Gene ID #:
3912
NCBI Official Name:
Laminin subunit beta-1
NCBI Official Symbol:
LAMB1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
198 kDa
Protein Accession #:
P07942
Protein GI Number:
317373377
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Neuroscience,Signal Transduction
Swissprot #:
P07942
User NOte:
Optimal dilutions for each application to be determined by the researcher.