VPS25 Antibody

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ProSci
Product Code: PSI-60-656
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-60-656-400ul400ul£626.00
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Application: Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in kidney cell line lysates (35ug/lane).
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Western blot analysis in kidney cell line lysates (35ug/lane).

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Further Information

Additional Names:
Vacuolar protein-sorting-associated protein 25, hVps25, Dermal papilla-derived protein 9, ELL-associated protein of 20 kDa, ESCRT-II complex subunit VPS25, VPS25, DERP9, EAP20
Application Note:
For WB starting dilution is: 1:1000
Background:
VPS25, VPS36 (MIM 610903), and SNF8 (MIM 610904) form ESCRT-II (endosomal sorting complex required for transport II), a complex involved in endocytosis of ubiquitinated membrane proteins. VPS25, VPS36, and SNF8 are also associated in a multiprotein complex with RNA polymerase II elongation factor (ELL; MIM 600284) (Slagsvold et al., 2005 [PubMed 15755741]; Kamura et al., 2001 [PubMed 11278625]).
Background References:
  • Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
  • Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)
  • Im, Y.J., et al. Dev. Cell 17(2):234-243(2009)
  • Lamesch, P., et al. Genomics 89(3):307-315(2007)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This VPS25 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 116-144 amino acids from the C-terminal region of human VPS25.
NCBI Gene ID #:
84313
NCBI Official Name:
Vacuolar protein-sorting-associated protein 25
NCBI Official Symbol:
VPS25
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
21 kDa
Protein Accession #:
Q9BRG1
Protein GI Number:
73920459
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
Q9BRG1
User NOte:
Optimal dilutions for each application to be determined by the researcher.