Mouse Aak1 Antibody

ProSci
Product Code: PSI-57-852
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-57-852-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in mouse bladder tissue lysates (35ug/lane).
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Mouse Aak1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Western blot analysis in mouse bladder tissue lysates (35ug/lane).
Mouse Aak1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Further Information

Additional Names:
AP2-associated protein kinase 1, Adaptor-associated kinase 1, Aak1, Kiaa1048
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
Aak1 phosphorylates the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2). May play a role in regulating aspects of clathrin-mediated endocytosis (By similarity).
Background References:
  • Munton, R.P., et al. Mol. Cell Proteomics 6(2):283-293(2007)
  • Nishimura, M., et al. DNA Res. 11(5):315-323(2004)
  • Jha, A., et al. J. Biol. Chem. 279(3):2281-2290(2004)
  • Zambrowicz, B.P., et al. Proc. Natl. Acad. Sci. U.S.A. 100(24):14109-14114(2003)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Human, Pig, Rat
Immunogen:
This Mouse Aak1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 279-306 amino acids from the Central region of mouse Aak1.
NCBI Gene ID #:
269774
NCBI Official Name:
AP2-associated protein kinase 1
NCBI Official Symbol:
Aak1
NCBI Organism:
Mus musculus
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
103 kDa
Protein Accession #:
Q3UHJ0
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
Q3UHJ0
User NOte:
Optimal dilutions for each application to be determined by the researcher.