Connexin 37 Antibody

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ProSci
Product Code: PSI-58-178
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-58-178-400ul400ul£626.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in mouse lung tissue lysates (35ug/lane).This demonstrates the GJA4 antibody detected the GJA4 protein (arrow).
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Connexin 37 Antibody (GJA4) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Connexin 37 Antibody (GJA4) for immunohistochemistry.
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Western blot analysis in mouse lung tissue lysates (35ug/lane).This demonstrates the GJA4 antibody detected the GJA4 protein (arrow).
Connexin 37 Antibody (GJA4) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Connexin 37 Antibody (GJA4) for immunohistochemistry.

Documents

Further Information

Additional Names:
Gap junction alpha-4 protein, Connexin-37, Cx37, GJA4
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
Gap junctions permit direct cell-to-cell passage of small cytoplasmic molecules, including ions, metabolic intermediates, and second messengers, and thereby mediate intercellular communication. Gap junction channels consist of connexin protein subunits encoded by a multigene family. Erythrokeratodermia variabilis (EKV) is an autosomal dominant disorder of keratinization characterized by migratory erythematous lesions and fixed keratotic plaques. Mutations in the GJB3 gene have been reported in some but not all families, although it has been postulated that the absence of connexin 30.3 can be compensated by other connexins.
Background References:
  • Strausberg, R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903 (2002).
  • Saito, T., et al., Int. J. Cancer 86(1):67-70 (2000).
  • Boerma, M., et al., J. Intern. Med. 246(2):211-218 (1999).
  • Krutovskikh, V., et al., Carcinogenesis 17(8):1761-1763 (1996).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This Connexin 37 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 303-333 amino acids from the C-terminal region of human Connexin 37.
NCBI Gene ID #:
2701
NCBI Official Name:
Gap junction alpha-4 protein
NCBI Official Symbol:
GJA4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
37 kDa
Protein Accession #:
P35212
Protein GI Number:
8928555
Purification:
This antibody is purified through a protein G column, followed by dialysis against PBS.
Research Area:
Signal Transduction
Swissprot #:
P35212
User NOte:
Optimal dilutions for each application to be determined by the researcher.