Mouse Pcp2 Antibody

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ProSci
Product Code: PSI-62-260
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-62-260-400ul400ul£626.00
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Application: Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Staining with Mouse Pcp2 Antibody was colocalized with GFP in most of the Purkinje cells with low background in 1:500 dilution condition. Intensity of PCP2 staining was not necessarily corresponded to the intensity of GFP, and there are a few GFP positive , not stained with pcp2 antibody, cells(see middle and low panel). In dendrites and axons of Purkinje cells, most of fibers stained with PCP-2 antibodies were colocalized with GFP.
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Staining with Mouse Pcp2 Antibody was colocalized with GFP in most of the Purkinje cells with low background in 1:500 dilution condition. Intensity of PCP2 staining was not necessarily corresponded to the intensity of GFP, and there are a few GFP positive , not stained with pcp2 antibody, cells(see middle and low panel). In dendrites and axons of Purkinje cells, most of fibers stained with PCP-2 antibodies were colocalized with GFP.

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Further Information

Additional Names:
Purkinje cell protein 2, Protein PCD-5, Purkinje cell-specific protein L7, Pcp2, Pcp-2
Application Note:
For IHC-P starting dilution is: 1:500
Background:
Pcp2 may function as a cell-type specific modulator for G protein-mediated cell signaling. This protein exhibits tissue specificity for cerebellum (Purkinje cells) and retinal bipolar neurons.
Background References:
  • Guan,J. et al. Biochem. J. 392 (PT 2), 389-397 (2005) Saito,H. et al. Biochem. Biophys. Res. Commun. 331 (4), 1216-1221 (2005) Redd,K.J. et al. J. Neurosci. Res. 70 (5), 631-637 (2002) Zhang,X. et al. Brain Res. Mol. Brain Res. 105 (1-2), 1-10 (2002) Luo,Y. et al. J. Biol. Chem. 274 (16), 10685-10688 (1999)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This Mouse Pcp2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 13-41 amino acids from the N-terminal region of mouse Pcp2.
NCBI Gene ID #:
18545
NCBI Official Name:
Purkinje cell protein 2
NCBI Official Symbol:
Pcp2
NCBI Organism:
Mus musculus
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
13 kDa
Protein Accession #:
P12660
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Swissprot #:
P12660
User NOte:
Optimal dilutions for each application to be determined by the researcher.