TAF1 Antibody
| Code | Size | Price |
|---|
| PSI-56-031-400ul | 400ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Application: Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
Transcription initiation factor TFIID subunit 1, Cell cycle gene 1 protein, TBP-associated factor 250 kDa, p250, Transcription initiation factor TFIID 250 kDa subunit, TAF(II)250, TAFII-250, TAFII250, TAF1, BA2R, CCG1, CCGS, TAF2A
Application Note:
For WB starting dilution is: 1:1000
Background:
Initiation of transcription by RNA polymerase II requires
the activities of more than 70 polypeptides. The protein that
coordinates these activities is the basal transcription factor
TFIID, which binds to the core promoter to position the polymerase
properly, serves as the scaffold for assembly of the remainder of
the transcription complex, and acts as a channel for regulatory
signals. TFIID is composed of the TATA-binding protein (TBP) and a
group of evolutionarily conserved proteins known as TBP-associated
factors or TAFs. TAFs may participate in basal transcription, serve
as coactivators, function in promoter recognition or modify general
transcription factors (GTFs) to facilitate complex assembly and
transcription initiation. This gene encodes the largest subunit of
TFIID. This subunit binds to core promoter sequences encompassing
the transcription start site. It also binds to activators and other
transcriptional regulators, and these interactions affect the rate
of transcription initiation. This subunit contains two independent
protein kinase domains at the N and C-terminals, but also possesses
acetyltransferase activity and can act as a
ubiquitin-activating/conjugating enzyme. This gene is part of a
complex transcriptional unit (TAF1/DYT3), wherein some products
share exons with TAF1 as well as additional exons
downstream.
Background References:
- Tavassoli, P., et al. Mol. Endocrinol. 24(4):696-708(2010)
- Allende-Vega, N., et al. Mol. Cell. Biochem. 316 (1-2), 99-106 (2008) :
- Centeno, F., et al. Intervirology 51(2):137-143(2008)
- Olejnik-Schmidt, A.K., et al. Arch. Virol. 153(5):983-990(2008)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This TAF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1863-1893 amino acids from the C-terminal region of human TAF1.
NCBI Gene ID #:
6872
NCBI Official Name:
Transcription initiation factor TFIID subunit 1
NCBI Official Symbol:
TAF1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
213 kDa
Protein Accession #:
P21675
Protein GI Number:
115942
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
P21675
User NOte:
Optimal dilutions for each application to be determined by the researcher.