MED14 Antibody
| Code | Size | Price |
|---|
| PSI-56-277-400ul | 400ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Application: Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
Mediator of RNA polymerase II transcription subunit 14, Activator-recruited cofactor 150 kDa component, ARC150, Cofactor required for Sp1 transcriptional activation subunit 2, CRSP complex subunit 2, Mediator complex subunit 14, RGR1 homolog, hRGR1, Thyroid hormone receptor-associated protein complex 170 kDa component, Trap170, Transcriptional coactivator CRSP150, Vitamin D3 receptor-interacting protein complex 150 kDa component, DRIP150, MED14
Application Note:
For WB starting dilution is: 1:1000
Background:
The activation of gene transcription is a multistep
process that is triggered by factors that recognize transcriptional
enhancer sites in DNA. These factors work with co-activators to
direct transcriptional initiation by the RNA polymerase II
apparatus. The protein encoded by this gene is a subunit of the
CRSP (cofactor required for SP1 activation) complex, which, along
with TFIID, is required for efficient activation by SP1. This
protein is also a component of other multisubunit complexes e.g.
thyroid hormone receptor-(TR-) associated proteins which interact
with TR and facilitate TR function on DNA templates in conjunction
with initiation factors and cofactors. This protein contains a
bipartite nuclear localization signal. This gene is known to escape
chromosome X-inactivation.
Background References:
- Wu, C., et al. Proteomics 7(11):1775-1785(2007)
- Lee, J., et al. Arch. Biochem. Biophys. 461(2):200-210(2007)
- Olsen, J.V., et al. Cell 127(3):635-648(2006)
- Olsen, J.V., et al. Cell 127(3):635-648(2006)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This MED14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 586-615 amino acids from the Central region of human MED14.
NCBI Gene ID #:
9282
NCBI Official Name:
Mediator of RNA polymerase II transcription subunit 14
NCBI Official Symbol:
MED14
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
161 kDa
Protein Accession #:
O60244
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
O60244
User NOte:
Optimal dilutions for each application to be determined by the researcher.