Mouse Cdkn2a Antibody
| Code | Size | Price |
|---|
| PSI-60-398-400ul | 400ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Application: Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
Cyclin-dependent kinase inhibitor 2A, isoform 3, p19ARF, Cdkn2a {ECO:0000312|EMBL:AAB357701, ECO:0000312|MGI:MGI:104738}
Application Note:
For WB starting dilution is: 1:1000
Background:
Capable of inducing cell cycle arrest in G1 and G2 phases. Acts as a tumor suppressor. Binds to MDM2 and blocks its nucleocytoplasmic shuttling by sequestering it in the nucleolus. This inhibits the oncogenic action of MDM2 by blocking MDM2-induced degradation of p53 and enhancing p53-dependent transactivation and apoptosis. Also induces G2 arrest and apoptosis in a p53-independent manner by preventing the activation of cyclin B1/CDC2 complexes. Binds to BCL6 and down-regulates BCL6-induced transcriptional repression. Binds to E2F1 and MYC and blocks their transcriptional activator activity but has no effect on MYC transcriptional repression. Binds to TOP1/TOPOI and stimulates its activity. This complex binds to rRNA gene promoters and may play a role in rRNA transcription and/or maturation. Interacts with NPM1/B23 and promotes its polyubiquitination and degradation, thus inhibiting rRNA processing. Interacts with UBE2I/UBC9 and enhances sumoylation of a number of its binding partners including MDM2 and E2F1. Binds to HUWE1 and represses its ubiquitin ligase activity. May play a role in controlling cell proliferation and apoptosis during mammary gland development.
Background References:
- Nogueira, C., et al. Oncogene 29(47):6222-6232(2010)
- Fernandez-Diaz, L.C., et al. Development 137(20):3393-3403(2010)
- Bennecke, M., et al. Cancer Cell 18(2):135-146(2010)
- Negishi, M., et al. PLoS ONE(8), E12373 (2010) :
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This Mouse Cdkn2a antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 39-65 amino acids from the Central region of mouse Cdkn2a.
NCBI Gene ID #:
12578
NCBI Official Name:
Cyclin-dependent kinase inhibitor 2A, isoform 3
NCBI Official Symbol:
Cdkn2a
NCBI Organism:
Mus musculus
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
19 kDa
Protein Accession #:
Q64364
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q64364
User NOte:
Optimal dilutions for each application to be determined by the researcher.