POLR3B Antibody
| Code | Size | Price |
|---|
| PSI-57-291-400ul | 400ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Application: Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
DNA-directed RNA polymerase III subunit RPC2, RNA polymerase III subunit C2, C128, DNA-directed RNA polymerase III 1276 kDa polypeptide, DNA-directed RNA polymerase III subunit B, POLR3B
Application Note:
For WB starting dilution is: 1:1000
Background:
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol III is composed of mobile elements and RPC2 is part of the core element with the central large cleft and probably a clamp element that moves to open and close the cleft (By similarity). Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF-Kappa-B through the RIG-I pathway.
Background References:
- Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
- Ablasser, A., et al. Nat. Immunol. 10(10):1065-1072(2009)
- Shen, E., et al. Exp. Cell Res. 315(7):1100-1116(2009)
- Yee, N.S., et al. PLoS Biol.(11), E312 (2007) :
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This POLR3B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 330-359 amino acids from the N-terminal region of human POLR3B.
NCBI Gene ID #:
55703
NCBI Official Name:
DNA-directed RNA polymerase III subunit RPC2
NCBI Official Symbol:
POLR3B
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
128 kDa
Protein Accession #:
Q9NW08
Protein GI Number:
29428029
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q9NW08
User NOte:
Optimal dilutions for each application to be determined by the researcher.