LARS Antibody

ProSci
Product Code: PSI-55-704
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-55-704-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
2 / 2
Western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).

Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).

Further Information

Additional Names:
Leucine--tRNA ligase, cytoplasmic, Leucyl-tRNA synthetase, LeuRS, LARS, KIAA1352
Application Note:
For FACS starting dilution is: 1:10~50

For WB starting dilution is: 1:1000
Background:
This gene encodes a cytosolic leucine-tRNA synthetase, a member of the class I aminoacyl-tRNA synthetase family. The encoded enzyme catalyzes the ATP-dependent ligation of L-leucine to tRNA(Leu). It is found in the cytoplasm as part of a multisynthetase complex and interacts with the arginine tRNA synthetase through its C-terminal domain. Alternatively spliced transcript variants of this gene have been found; however, their full-length nature is not known.
Background References:
  • Pang, Y.L., et al. Biochemistry 48(38):8958-8964(2009)
  • Seiradake, E., et al. J. Mol. Biol. 390(2):196-207(2009)
  • Shin, S.H., et al. Exp. Mol. Med. 40(2):229-236(2008)
  • Maeso, E., et al. Neuromuscul. Disord. 17(5):415-418(2007)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This LARS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 176-205 amino acids from the N-terminal region of human LARS.
NCBI Gene ID #:
51520
NCBI Official Name:
Leucine--tRNA ligase, cytoplasmic
NCBI Official Symbol:
LARS
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
134 kDa
Protein Accession #:
Q9P2J5
Protein GI Number:
48428689
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Swissprot #:
Q9P2J5
User NOte:
Optimal dilutions for each application to be determined by the researcher.