TFIIIC90 Antibody

ProSci
Product Code: PSI-56-142
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-56-142-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).
2 / 2
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Further Information

Additional Names:
General transcription factor 3C polypeptide 4, TF3C-delta, Transcription factor IIIC 90 kDa subunit, TFIIIC 90 kDa subunit, TFIIIC90, Transcription factor IIIC subunit delta, GTF3C4
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
Essential for RNA polymerase III to make a number of small nuclear and cytoplasmic RNAs, including 5S RNA, tRNA, and adenovirus-associated (VA) RNA of both cellular and viral origin. Has histone acetyltransferase activity (HAT) with unique specificity for free and nucleosomal H3. May cooperate with GTF3C5 in facilitating the recruitment of TFIIIB and RNA polymerase through direct interactions with BRF1, POLR3C and POLR3F. May be localized close to the A box.
Background References:
  • Joubert, B.R., et al. Genome Med(3), 17 (2010) :
  • Dumay-Odelot, H., et al. J. Biol. Chem. 282(23):17179-17189(2007)
  • Olsen, J.V., et al. Cell 127(3):635-648(2006)
  • Beausoleil, S.A., et al. Proc. Natl. Acad. Sci. U.S.A. 101(33):12130-12135(2004)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This TFIIIC90 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 214-244 amino acids from the N-terminal region of human TFIIIC90.
NCBI Gene ID #:
9329
NCBI Official Name:
General transcription factor 3C polypeptide 4
NCBI Official Symbol:
GTF3C4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
92 kDa
Protein Accession #:
Q9UKN8
Protein GI Number:
212276467
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q9UKN8
User NOte:
Optimal dilutions for each application to be determined by the researcher.