LIMD1 Antibody

ProSci
Product Code: PSI-56-870
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-56-870-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in mouse brain tissue lysates (35ug/lane).
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LIMD1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Western blot analysis in mouse brain tissue lysates (35ug/lane).
LIMD1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Further Information

Additional Names:
LIM domain-containing protein 1, LIMD1
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
LIMD1 is a transcriptional regulator that inhibits E2F-mediated transcription, and suppresses the expression of the majority of genes with E2F1-responsive elements. May act as a tumor suppressor by inhibiting cell proliferation.
Background References:
  • Ghosh, S., et al. Mol. Cancer 9, 58 (2010) :
  • Sharp, T.V., et al. Proc. Natl. Acad. Sci. U.S.A. 105(50):19932-19937(2008)
  • Spendlove, I., et al. Int. J. Cancer 123(10):2247-2253(2008)
  • Huggins, C.J., et al. Cancer Lett. 267(1):55-66(2008)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Rat
Immunogen:
This LIMD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 438-467 amino acids from the C-terminal region of human LIMD1.
NCBI Gene ID #:
8994
NCBI Official Name:
LIM domain-containing protein 1
NCBI Official Symbol:
LIMD1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
72 kDa
Protein Accession #:
Q9UGP4
Protein GI Number:
47605932
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q9UGP4
User NOte:
Optimal dilutions for each application to be determined by the researcher.