PHF12 Antibody

ProSci
Product Code: PSI-55-655
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-55-655-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in mouse lung tissue lysates (35ug/lane).
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PHF12 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Western blot analysis in mouse lung tissue lysates (35ug/lane).
PHF12 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Further Information

Additional Names:
PHD finger protein 12, PHD factor 1, Pf1, PHF12 (HGNC:20816)
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins.
Background References:
  • Nousiainen, M., et al. Proc. Natl. Acad. Sci. U.S.A. 103(14):5391-5396(2006)
  • Nousiainen, M., et al. Proc. Natl. Acad. Sci. U.S.A. 103(14):5391-5396(2006)
  • Beausoleil, S.A., et al. Proc. Natl. Acad. Sci. U.S.A. 101(33):12130-12135(2004)
  • Yochum, G.S., et al. Mol. Cell. Biol. 22(22):7868-7876(2002)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This PHF12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 831-859 amino acids from the C-terminal region of human PHF12.
NCBI Gene ID #:
57649
NCBI Official Name:
PHD finger protein 12
NCBI Official Symbol:
PHF12
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
110 kDa
Protein Accession #:
Q96QT6
Protein GI Number:
71153050
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q96QT6
User NOte:
Optimal dilutions for each application to be determined by the researcher.