Tiparp Antibody

ProSci
Product Code: PSI-63-317
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-63-317-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with Tiparp antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
2 / 2
Western blot analysis in mouse spleen tissue lysates (35ug/lane).

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with Tiparp antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis in mouse spleen tissue lysates (35ug/lane).

Further Information

Additional Names:
TCDD-inducible poly [ADP-ribose] polymerase, ADP-ribosyltransferase diphtheria toxin-like 14, ARTD14, Tiparp
Application Note:
For IHC-P starting dilution is: 1:10~50

For WB starting dilution is: 1:1000
Background:
Poly [ADP-ribose] polymerase using NAD(+) as a substrate to transfer ADP-ribose onto glutamic acid residues of a protein acceptor; repeated rounds of ADP-ribosylation leads to the formation of poly(ADPribose) chains on the protein, thereby altering the function of the target protein. May play a role in the adaptative response to chemical exposure (TCDD) and thereby mediates certain effects of the chemicals.
Background References:
  • Schmahl,J., Nat. Genet. 39 (1), 52-60 (2007)
  • Ma,Q., Arch. Biochem. Biophys. 404 (2), 309-316 (2002)
  • Ma,Q., Biochem. Biophys. Res. Commun. 289 (2), 499-506 (2001)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This Tiparp antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 60-90 amino acids from the N-terminal region of human Tiparp.
NCBI Gene ID #:
99929
NCBI Official Name:
TCDD-inducible poly [ADP-ribose] polymerase
NCBI Official Symbol:
Tiparp
NCBI Organism:
Mus musculus
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
76 kDa
Protein Accession #:
Q8C1B2
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Swissprot #:
Q8C1B2
User NOte:
Optimal dilutions for each application to be determined by the researcher.