EIF2S1 Antibody

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ProSci
Product Code: PSI-57-063
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-57-063-400ul400ul£626.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in mouse bladder tissue lysates (35ug/lane).
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Confocal immunofluorescent analysis of EIF2S1 Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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Western blot analysis in mouse bladder tissue lysates (35ug/lane).
Confocal immunofluorescent analysis of EIF2S1 Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Documents

Further Information

Additional Names:
Eukaryotic translation initiation factor 2 subunit 1, Eukaryotic translation initiation factor 2 subunit alpha, eIF-2-alpha, eIF-2A, eIF-2alpha, EIF2S1, EIF2A
Application Note:
For WB starting dilution is: 1:1000

For IF starting dilution is: 1:10~50

For IHC-P starting dilution is: 1:10~50
Background:
The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).
Background References:
  • Shiota, M., et al. Int. J. Oncol. 36(6):1521-1531(2010)
  • Kulkarni, A.P., et al. Indian J. Biochem. Biophys. 47(2):67-74(2010)
  • Wehner, K.A., et al. Mol. Cell. Biol. 30(8):2006-2016(2010)
  • Backes, S., et al. J. Gen. Virol. 91 (PT 2), 470-482 (2010) :
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Rat, Chicken, Yeast, Bovine, Drosophila
Immunogen:
This EIF2S1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 60-89 amino acids from the N-terminal region of human EIF2S1.
NCBI Gene ID #:
1965
NCBI Official Name:
Eukaryotic translation initiation factor 2 subunit 1
NCBI Official Symbol:
EIF2S1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
36 kDa
Protein Accession #:
P05198
Protein GI Number:
124200
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
P05198
User NOte:
Optimal dilutions for each application to be determined by the researcher.