BAAT Antibody
| Code | Size | Price |
|---|
| PSI-61-279-200ul | 200ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Rat
Application: Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
Bile acid-CoA:amino acid N-acyltransferase, BACAT, BAT, 2.3.1.65, Glycine N-choloyltransferase, Long-chain fatty-acyl-CoA hydrolase, 3.1.2.2, BAAT
Application Note:
For WB starting dilution is: 1:2000
Background:
Involved in bile acid metabolism. In liver hepatocytes catalyzes the second step in the conjugation of C24 bile acids (choloneates) to glycine and taurine before excretion into bile canaliculi. The major components of bile are cholic acid and chenodeoxycholic acid. In a first step the bile acids are converted to an acyl-CoA thioester, either in peroxisomes (primary bile acids deriving from the cholesterol pathway), or cytoplasmic at the endoplasmic reticulum (secondary bile acids). May catalyze the conjugation of primary or secondary bile acids, or both. The conjugation increases the detergent properties of bile acids in the intestine, which facilitates lipid and fat-soluble vitamin absorption. In turn, bile acids are deconjugated by bacteria in the intestine and are recycled back to the liver for reconjugation (secondary bile acids). May also act as an acyl-CoA thioesterase that regulates intracellular levels of free fatty acids. In vitro, catalyzes the hydrolysis of long- and very long-chain saturated acyl-CoAs to the free fatty acid and coenzyme A (CoASH), and conjugates glycine to these acyl-CoAs.
Background References:
- Falany C.N.,et al.J. Biol. Chem. 269:19375-19379(1994).
- Ebert L.,et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
- Humphray S.J.,et al.Nature 429:369-374(2004).
- Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This BAAT antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 63-95 amino acids from human BAAT.
NCBI Gene ID #:
570
NCBI Official Name:
Bile acid-CoA:amino acid N-acyltransferase
NCBI Official Symbol:
BAAT
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
46 kDa
Protein Accession #:
Q14032
Protein GI Number:
74739811
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Obesity,Signal Transduction
Swissprot #:
Q14032
User NOte:
Optimal dilutions for each application to be determined by the researcher.