UcH-L3 (human) (rec.) (untagged)

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South Bay Bio
Product Code: SBB-DE0023
Product Group: Recombinant Proteins
Supplier: South Bay Bio
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SBB-DE0023-C05050 ug£275.00
Quantity:
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Overview

Regulatory Status: RUO
Target Species: Human
Shipping:
Dry Ice
Storage:
-80C

Images

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Michaelis?Menten Kinetics: Ubiquitin Rhodamine 110 serially diluted from 1.6 to 0.05?M was digested with 30pM UcHL3 over time. The assay was carried out in a reaction buffer of 50mM HEPES pH 7.5, 100mM NaCl, 1mM TCEP, 0.1mg/ml BSA, at 25?C. Initial
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Michaelis?Menten Kinetics: Ubiquitin Rhodamine 110 serially diluted from 1.6 to 0.05?M was digested with 30pM UcHL3 over time. The assay was carried out in a reaction buffer of 50mM HEPES pH 7.5, 100mM NaCl, 1mM TCEP, 0.1mg/ml BSA, at 25?C. Initial

Documents

Further Information

Alternate Names/Synonyms:
Ubiquitin C-terminal Hydrolase L3; Ubiquitin Thioesterase L3
Concentration:
Lot dependent.
EClass:
32160000
Form (Short):
liquid
Formulation:
Liquid. In 50mM HEPES pH 7.5, 100mM sodium chloride, 1mM TCEP.
Handling Advice:
Aliquot to avoid freeze/thaw cycles.
Long Description:
Protein. Human UcH-L3 (aa 1-230)is untagged. Source: E. coli. Formulation: Liquid. In 50mM HEPES pH 7.5, 100mM sodium chloride, 1mM TCEP. Purity: >95% (SDS-PAGE). UCHL3 (Ubiquitin C-terminal Hydrolase L3) is a deubiquitinating enzyme (DUB) that controls levels of cellular ubiquitin through processing of ubiquitin precursors and ubiquitinated proteins. It is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. It plays a role in regulating apical membrane recycling and indirectly increases the phosphorylation of IGFIR, AKT and FOXO1 and promotes insulin-signaling and insulin-induced adipogenesis. It is required for stress-response in retinal, skeletal muscle and germ cell maintenance. UCHL3 is also known to hydrolyze UBB(+1), a mutated form of ubiquitin which is not effectively degraded by the proteasome and is associated with neurogenerative disorders. It digests precursors and ubiquitinated proteins to generate monomeric ubiquitin.
Molecular Weight:
~26kDa
NCBI, Uniprot Number:
P15374
Package Type:
Plastic Vial
Product Description:
UCHL3 (Ubiquitin C-terminal Hydrolase L3) is a deubiquitinating enzyme (DUB) that controls levels of cellular ubiquitin through processing of ubiquitin precursors and ubiquitinated proteins. It is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. It plays a role in regulating apical membrane recycling and indirectly increases the phosphorylation of IGFIR, AKT and FOXO1 and promotes insulin-signaling and insulin-induced adipogenesis. It is required for stress-response in retinal, skeletal muscle and germ cell maintenance. UCHL3 is also known to hydrolyze UBB(+1), a mutated form of ubiquitin which is not effectively degraded by the proteasome and is associated with neurogenerative disorders. It digests precursors and ubiquitinated proteins to generate monomeric ubiquitin.
Purity:
>95% (SDS-PAGE)
Sequence:
Human UcH-L3 (aa 1-230) (Accession Nr. P15374 http://www.ncbi.nlm.nih.gov/protein/P15374 ) is untagged.
Source / Host:
E. coli
TAGs:
no TAG
Transportation:
Non-hazardous
UNSPSC Category:
Other Proteins
UNSPSC Number:
12352202
Use & Stability:
Stable for at least 1 year after receipt when stored at -80°C.