Ubiquitin vinyl methyl ester (human) (rec.)

Lot dependent.

32160000

liquid

Liquid. In 50mM MES pH 6.0, 100mM sodium chloride.

Aliquot to avoid freeze/thaw cycles.

Protein. Human ubiquitin (aa1-76)with a C-terminal vinyl methyl ester group. Source: E. coli. Formulation: Liquid. In 50mM MES pH 6.0, 100mM sodium chloride. Purity: >97% (LCMS). Ubiquitin is a small (8.5kDa) regulatory protein that has been found in almost all tissues of eukaryotic organisms. The addition of ubiquitin to a substrate protein is called ubiquitination or ubiquitylation. Ubiquitination can affect proteins in many ways: it can signal for their degradation via the proteasome, alter their cellular location, affect their activity and promote or prevent protein interactions. Removal of ubiquitin from a substrate protein occurs via deconjugating enzymes, of which there are nearly 100 known enzymes with various linkage specificities. This product consists of a full-length human, mature ubiquitin polypeptide (amino acids 1-76), expressed in E.coli with a C-terminal warhead (Vinyl Methyl Ester). Ubiquitin vinyl methyl ester is a potent, irreversible and specific inhibitor of deubiquitinating enzymes (DUBs) based on a C-terminal electrophilic vinyl methyl ester group. Ubiquitin vinyl methyl ester can be used for activity profiling experiments and determining DUB inhibitor specificity. It targets three of the four major DUB families: UCH (Ubiquitin C-terminal hydrolases), USP (Ubiquitin specific proteases), OTU (Ovarian tumor proteases) and MJD (Machado-Josephin domain proteases) while JAMM metalloproteases are not inhibited.

~9kDa

P0CG47

Plastic Vial

Ubiquitin is a small (8.5kDa) regulatory protein that has been found in almost all tissues of eukaryotic organisms. The addition of ubiquitin to a substrate protein is called ubiquitination or ubiquitylation. Ubiquitination can affect proteins in many ways: it can signal for their degradation via the proteasome, alter their cellular location, affect their activity and promote or prevent protein interactions. Removal of ubiquitin from a substrate protein occurs via deconjugating enzymes, of which there are nearly 100 known enzymes with various linkage specificities. This product consists of a full-length human, mature ubiquitin polypeptide (amino acids 1-76), expressed in E.coli with a C-terminal warhead (Vinyl Methyl Ester). Ubiquitin vinyl methyl ester is a potent, irreversible and specific inhibitor of deubiquitinating enzymes (DUBs) based on a C-terminal electrophilic vinyl methyl ester group. Ubiquitin vinyl methyl ester can be used for activity profiling experiments and determining DUB inhibitor specificity. It targets four of the five major DUB families: UCH (Ubiquitin C-terminal hydrolases), USP (Ubiquitin specific proteases), OTU (Ovarian tumor proteases) and MJD (Machado-Josephin domain proteases) while JAMM metalloproteases are not inhibited.

>97% (LCMS)

Human ubiquitin (aa1-76) (Accession Nr. P0CG47 http://www.ncbi.nlm.nih.gov/protein/P0CG47 ) with a C-terminal vinyl methyl ester group.

E. coli

Non-hazardous

Other Proteins

12352202

Stable for at least 1 year after receipt when stored at -80°C.