LIGHT Antibody [8D4]

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ProSci
Product Code: PSI-RF16064
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-RF16064-0.02mg0.02mg£150.00
Quantity:
PSI-RF16064-0.1mg0.1mg£515.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG2b
Antibody Clonality: Monoclonal
Antibody Clone: 8D4
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Flow Cytometry
  • Immunocytochemistry (ICC)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Shipping:
blue ice
Storage:
LIGHT antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Images

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Western blot analysis of LIGHT in overexpressing HEK293 cells with LIGHT antibody at 0.5 and 1 μg/ml
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Immunocytochemistry of LIGHT in transfected HEK293 cells with LIGHT antibody at 1 μg/mL. Lower left: Immunocytochemistry in transfected HEK293 cells with control mouse IgG antibody at 1 μg/mL.
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Immunofluorescence of LIGHT in transfected HEK293 cells with LIGHT antibody at 2 μg/mL. <br><br>Green: LIGHT Antibody [8D4] (RF16064) <br> Blue: DAPI staining
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Immunofluorescence of LIGHT in human lymphoma tissue with LIGHT antibody at 20 μg/mL. <br><br>Green: LIGHT Antibody [8D4] (RF16064) <br> Blue: DAPI staining
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Immunohistochemistry of LIGHT in human lymphoma tissue with LIGHT antibody at 5 μg/mL.
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Flow cytometry analysis of LIGHT overexpressing HEK293 cells using LIGHT antibody and control mouse IgG antibody at 10 μg/ml. Blue: Untransfected HEK293 cells. Yellow: LIGHT overexpressing HEK293 cells.
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Western blot analysis of LIGHT in overexpressing HEK293 cells with LIGHT antibody at 0.5 and 1 μg/ml
Immunocytochemistry of LIGHT in transfected HEK293 cells with LIGHT antibody at 1 μg/mL. Lower left: Immunocytochemistry in transfected HEK293 cells with control mouse IgG antibody at 1 μg/mL.
Immunofluorescence of LIGHT in transfected HEK293 cells with LIGHT antibody at 2 μg/mL. <br><br>Green: LIGHT Antibody [8D4] (RF16064) <br> Blue: DAPI staining
Immunofluorescence of LIGHT in human lymphoma tissue with LIGHT antibody at 20 μg/mL. <br><br>Green: LIGHT Antibody [8D4] (RF16064) <br> Blue: DAPI staining
Immunohistochemistry of LIGHT in human lymphoma tissue with LIGHT antibody at 5 μg/mL.
Flow cytometry analysis of LIGHT overexpressing HEK293 cells using LIGHT antibody and control mouse IgG antibody at 10 μg/ml. Blue: Untransfected HEK293 cells. Yellow: LIGHT overexpressing HEK293 cells.

Documents

Further Information

Additional Names:
LIGHT Antibody: TNFSF14, LTg, CD258, HVEML, HVEM ligand
Application Note:
LIGHT antibody can be used for detection of CD80 by Western blot at 0.5 - 1 μg/mL. LIGHT antibody can be used for immunohistochemistry starting at 2 - 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples; Immunofluorescence in human samples and Flow Cytometry in mouse samples. All other applications and species not yet tested.
Background:
LIGHT Antibody: LIGHT, also known as Tumor Necrosis Factor Superfamily member 14 (TNFSF14), is a co-stimulatory molecule that can regulate T-cell activation (1) and has recently been identified as an immune checkpoint protein. LIGHT binds to two different receptors, Herpes Virus Entry Mediator (HVEM) and Lymphotoxin beta Receptor (LT?R). While LIGHT binding to HVEM delivers a co-stimulatory signal to T cells (1), LIGHT binding to LT?R is critical for the formation of lymphoid structures which can stimulate T cell infiltration and activation of a tumor microenvironment, leading to rapid T cell-mediated tissue destruction (2). It has been shown that targeted delivery of LIGHT to tumors, thereby causing the T cell infiltration of the tumor, can enhance the response of the PD-1/PD-L1 checkpoint blockade anti-cancer therapy (3), suggesting that LIGHT may become a potent tool in anti-cancer treatment.
Background References:
  • Wang Y, Zhu M, Miller M, et al. Immunoregulation by tumor necrosis factor superfamily member LIGHT. Immunological Reviews 2009; 229:232?43.
  • Lee Y, Chin RK, Christiansen P, et al. Recruitment and activation of naive T cells in the islets by lymphotoxin beta receptor-dependent tertiary lymphoid structure. Immunity 2006; 25:499-509.
  • Tang H, Wang Y, Chlewicki LK, et al. Facilitating T cell infiltration in tumor microenvironment overcomes resistance to PD-L1 blockade. Cancer Cell 2016; 29:285-296.
Buffer:
LIGHT Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
LIGHT antibody was raised against the extracellular domain of human LIGHT.
NCBI Gene ID #:
8740
NCBI Official Name:
TNF superfamily member 14
NCBI Official Symbol:
TNFSF14
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 26 kDa

Protein Accession #:
NP_003798
Protein GI Number:
25952144
Purification:
LIGHT Antibody is supplied as protein A purified IgG2b.
Research Area:
Immunology
Swissprot #:
O43557
User NOte:
Optimal dilutions for each application to be determined by the researcher.