5-Carboxy-2',7'-dichlorofluorescein DA

5-CDCFDA; 5-Carboxy-2',7'-dichlorofluorescein diacetate; 5-Carboxy-2',7'-dichlorofluorescein DA

Off-white powder.

144489-09-8

32160000

liquid

Keep cool and dry.Protect from light and moisture.

InChI=1S/C25H14Cl2O9/c1-10(28)33-21-8-19-15(6-17(21)26)25(14-4-3-12(23(30)31)5-13(14)24(32)36-25)16-7-18(27)22(34-11(2)29)9-20(16)35-19/h3-9H,1-2H3,(H,30,31)

AJIVVDSJIOOZOJ-UHFFFAOYSA-N

Chemical. CAS: 144489-09-8. Formula: C25H14Cl2O9. MW: 529.28. Synthetic. Non-activated, amine-reactive fluorescent probe. Single 5-isomer of CDCFDA that can be used in biomolecules conjugation. CDCFDA is an amine?reactive fluorescein diacetate (FDA) derivative used to prepare a variety of FDA conjugates. CDCFDA is a non-fluorescent molecule that diffuse into cells and is hydrolyzed by intracellular non-specific esterases, yielding a fluorescent probe. The probe accumulates exclusively in viable cells with intact cell membranes. CFCFDA does not stain dead cells. CDCFDA labeling can be used for monitoring cells by flow cytometry or fluorescence microscopy. The probe is also frequently used to monitor reactive oxygen species (ROS) generation in live cells and intracellular pH levels. Spectral data: lambdaex=504nm, lambdaem=529nm.

MFCD00037454

C25H14Cl2O9

529.28

Vial

Non-activated, amine-reactive fluorescent probe. Single 5-isomer of CDCFDA that can be used in biomolecules conjugation. CDCFDA is an amine?reactive fluorescein diacetate (FDA) derivative used to prepare a variety of FDA conjugates. CDCFDA is a non-fluorescent molecule that diffuse into cells and is hydrolyzed by intracellular non-specific esterases, yielding a fluorescent probe. The probe accumulates exclusively in viable cells with intact cell membranes. CFCFDA does not stain dead cells. CDCFDA labeling can be used for monitoring cells by flow cytometry or fluorescence microscopy. The probe is also frequently used to monitor reactive oxygen species (ROS) generation in live cells and intracellular pH levels. Spectral data: lambdaex=504nm, lambdaem=529nm.

>95% (NMR)

ClC1=C(OC(C)=O)C=C2C(C3(C(C=CC(C(O)=O)=C4)=C4C(O3)=O)C(C=C(Cl)C(OC(C)=O)=C5)=C5O2)=C1

Soluble in DMSO, DMF, acetonitrile, methanol or chloroform.

Synthetic.

Non-hazardous

Fluorescent Reagents

41105331

Stable for at least 2 years after receipt when stored at -20°C.