Fluorescein diacetate

FDA; 3,6-Diacetoxyfluoran; Di-O-Acetylfluorescein; Cellstain-FDA; NSC 4726; NSC 667259

White to off-white powder.

596-09-8

32160000

liquid

Keep cool and dry.Protect from light and moisture.

InChI=1S/C24H16O7/c1-13(25)28-15-7-9-19-21(11-15)30-22-12-16(29-14(2)26)8-10-20(22)24(19)18-6-4-3-5-17(18)23(27)31-24/h3-12H,1-2H3

CHADEQDQBURGHL-UHFFFAOYSA-N

Chemical. CAS: 596-09-8. Formula: C24H16O7. MW: 416.38. Synthetic. FDA is a non-fluorescent hydrophobic fluorescein derivative that can pass through the cell membrane whereupon intracellular esterases hydrolyze the diacetate group producing the highly fluorescent product fluorescein. The fluorescein molecules accumulate in cells that possess intact membranes so the green fluorescence can be used as a marker of cell viability. Cells that do not possess an intact cell membrane or an active metabolism may not accumulate the fluorescent product and therefore do not exhibit green fluorescence. FDA may be used in combination with PI staining as the non-viable cells take up the PI and stain dead cells red whereas viable cells do not take up the PI and should only stain green. This 2-color separation of non-viable and viable cells may provide a more accurate quantitation of cell viability than single color analysis. FDA is used in studies on intracellular interactions and membrane permeability, for flow cytometry and as esterase substrate. Spectral data: lambdaex=494nm, lambdaem=521nm.

MFCD00005062

C24H16O7

416.38

Vial

FDA is a non-fluorescent hydrophobic fluorescein derivative that can pass through the cell membrane whereupon intracellular esterases hydrolyze the diacetate group producing the highly fluorescent product fluorescein. The fluorescein molecules accumulate in cells that possess intact membranes so the green fluorescence can be used as a marker of cell viability. Cells that do not possess an intact cell membrane or an active metabolism may not accumulate the fluorescent product and therefore do not exhibit green fluorescence. FDA may be used in combination with PI staining as the non-viable cells take up the PI and stain dead cells red whereas viable cells do not take up the PI and should only stain green. This 2-color separation of non-viable and viable cells may provide a more accurate quantitation of cell viability than single color analysis. FDA is used in studies on intracellular interactions and membrane permeability, for flow cytometry and as esterase substrate. Spectral data: lambdaex=494nm, lambdaem=521nm.

>95% (NMR)

O=C(C1=C2C=CC=C1)OC32C4=C(C=C(OC(C)=O)C=C4)OC5=CC(OC(C)=O)=CC=C53

Soluble in DMSO, ethanol, water, chloroform or acetone (25mg/ml).

Synthetic.

Non-hazardous

Fluorescent Reagents

41105331

Stable for at least 2 years after receipt when stored at -20°C.