Gremlin Recombinant Protein
| Code | Size | Price |
|---|
| PSI-96-881-0.1mg | 0.1mg | £657.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Lyophilized Protein should be stored at -20˚C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚C or -70˚C. Avoid repeated freeze-thaw cycles.
Documents
Further Information
Additional Names:
GREM1,CKTSF1B1,DAND2,DRM,PIG2
Application Note:
This recombinant protein can be used for E, WB. For research use only.
Background:
Gremlin is also known as Cysteine knot superfamily 1, BMP antagonist 1 (CKTSF1B1), DAN domain family member 2 (DAND2), Down-regulated in Mos-transformed cells protein (DRM), Increased in high glucose protein 2 (IHG-2), Cell proliferation-inducing gene 2 protein (PIG2) or Gremlin-1 (GREM1), which is highly expressed in small intestine, fetal brain and colon. Gremlin / GREM-1 interacts with SLIT1 and SLIT2 in a glycosylation-dependent manner. Gremlin may play an important role during carcinogenesis and metanephric kidney organogenesis, as a BMP antagonist required for early limb outgrowth and patterning in maintaining the FGF4-SHH feedback loop. Gremlin down-regulates the BMP4 signaling in a dose-dependent manner and acts as inhibitor of monocyte chemotaxis.
Background References:
- McMahon R., et al., 2000, J. Biol. Chem. 275:9901-9904.
- Topol L.Z., et al., 2000, Cytogenet. Cell Genet. 89:79-84.
- Jaeger E., et al., 2012, Nat. Genet. 44:699-703.
Biological Activity:
Measured by its binding ability in a functional ELISA. Immobilized Human BMP-2, Tag Free at 2 ug/mL (100 uL/well) can bind Human Gremlin, Fc Tag with a linear range of 0.8-80 ng/mL.
Buffer:
50 mM Tris, 150 mM NaCl, pH7.5 with 1 mM EDTA
Fusion Tag:
Fc Tag
NCBI Gene ID #:
26585
NCBI Official Name:
gremlin-1 isoform 1 precursor
NCBI Official Symbol:
Gremlin
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Predicted Molecular Weight:
44.2 kDa
Protein Accession #:
NP_037504
Purity:
>95% as determined by SDS-PAGE.
Endotoxin level is less than 1.0 EU per ug by the LAL method.
Endotoxin level is less than 1.0 EU per ug by the LAL method.
Recombinant Protein Sequence:
Thr32-Gly402
Source:
HEK293 cells
Swissprot #:
O60565