TNF-alpha Recombinant Protein
| Code | Size | Price |
|---|
| PSI-96-958-0.1mg | 0.1mg | £657.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Lyophilized Protein should be stored at -20˚C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚C or -70˚C. Avoid repeated freeze-thaw cycles.
Documents
Further Information
Additional Names:
DIF,TNF-alpha,TNFA,TNFSF2,cachexin,cachectin,TNFalpha
Application Note:
This recombinant protein can be used for E, WB. For research use only.
Background:
Tumor necrosis factor alpha (TNFα) is a cytokine produced primarily by monocytes and macrophages. It is found in synovial cells and macrophages in the tissues.The primary role of TNFα is in the regulation of immune cells. TNFα is able to induce apoptotic cell death, to induce inflammation, and to inhibit tumorigenesis and viral replication. Dysregulation of TNFα production has been implicated in a variety of human diseases, including major depression, Alzheimer's disease and cancer. Recombinant TNFα is used as an immunostimulant under the INN tasonermin. TNFα can be produced ectopically in the setting of malignancy and parallels parathyroid hormone both in causing secondary hypercalcemia and in the cancers with which excessive production is associated.
Background References:
- Dowlati Y, Herrmann N, et al., 2010, Biol Psychiatry 67 (5): 446?457.
- Swardfager W, Lanctot K, et al., 2010, Biol Psychiatry 68 (10): 930?941.
- Locksley RM, Killeen N et al., 2001, Cell 104 (4): 487?501.
Biological Activity:
Measured by its binding ability in a functional ELISA. Immobilized Human TNF-alpha, His Tag at 0.2 ug/mL (100 uL/well) can bind Human TNFR1, Fc Tag with a linear range of 0.4-6.25 ng/mL.
Buffer:
PBS, pH7.4
Fusion Tag:
His Tag
NCBI Gene ID #:
7124
NCBI Official Name:
Tumor necrosis factor
NCBI Official Symbol:
TNF-alpha
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Predicted Molecular Weight:
18.2 kDa
Protein Accession #:
NP_000585.2
Purity:
>95% as determined by SDS-PAGE.
Endotoxin level is less than 1.0 EU per ug by the LAL method.
Endotoxin level is less than 1.0 EU per ug by the LAL method.
Recombinant Protein Sequence:
Thr32-Gly402
Source:
HEK293 cells
Swissprot #:
N/A