PDGF-B Recombinant Protein
| Code | Size | Price |
|---|
| PSI-96-936-0.05mg | 0.05mg | £657.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Lyophilized Protein should be stored at -20˚C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚C or -70˚C. Avoid repeated freeze-thaw cycles.
Documents
Further Information
Additional Names:
PDGFBB,PDGFB,FLJ12858,PDGF2,SIS,SSV,c-sis
Application Note:
This recombinant protein can be used for E, WB. For research use only.
Background:
PDGFs are mitogenic during early developmental stages, driving the proliferation of undifferentiated mesenchyme and some progenitor populations. During later maturation stages, PDGF signalling has been implicated in tissue remodelling and cellular differentiation, and in inductive events involved in patterning and morphogenesis. In addition to driving mesenchymal proliferation, PDGFs have been shown to direct the migration, differentiation and function of a variety of specialised mesenchymal and migratory cell types, both during development and in the adult animal. Other growth factors in this family include vascular endothelial growth factors B and C (VEGF-B, VEGF-C)which are active in angiogenesis and endothelial cell growth, and placenta growth factor (PlGF) which is also active in angiogenesis. PDGF plays a role in embryonic development, cell proliferation, cell migration, and angiogenesis. PDGF is a required element in cellular division for fibroblast, a type of connective tissue cell. PDGF is also known to maintain proliferation of oligodendrocyte progenitor cells. Platelet-derived growth factor subunit B is also known as PDGFB, FLJ12858, PDGF2, SIS, SSV, c-sis, is a member of the platelet-derived growth factor family. PDGFB can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma.
Background References:
- Hoch RV, Soriano P. 2003, Development 130 (20): 4769?4784.
- Olofsson B, et al. 1996, Proc Natl Acad Sci U S A.,93(6):2576-81.
- Joukov V, et al. 1996, EMBO J. 15 (2): 290?298.
- Lei KJ, et al. 1993, Oncogene 8 (4): 925?931.
Biological Activity:
Measured by its binding ability in a functional ELISA. Immobilized Human PDGF-BB, His Tag at 5 ug/mL (100 ul/well) can bind Human PDGF R beta, Fc Tag with a linear range of 0.02-1.2 ug/mL.
Buffer:
0.085% TFA in 30% ACN
Fusion Tag:
His Tag & Avi Tag
NCBI Gene ID #:
5155
NCBI Official Name:
Platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog)
NCBI Official Symbol:
PDGF-B
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Predicted Molecular Weight:
15 kDa
Protein Accession #:
AAH29822
Purity:
>95% as determined by SDS-PAGE.
Endotoxin level is less than 1.0 EU per ug by the LAL method.
Endotoxin level is less than 1.0 EU per ug by the LAL method.
Recombinant Protein Sequence:
Thr32-Gly402
Source:
E. coli
Swissprot #:
P01127