Carbonic Anhydrase IX Recombinant Protein
| Code | Size | Price |
|---|
| PSI-96-837-0.05mg | 0.05mg | £746.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
Lyophilized Protein should be stored at -20˚C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚C or -70˚C. Avoid repeated freeze-thaw cycles.
Documents
Further Information
Additional Names:
CAIX,CA9,CA-IX,G250,MN,P54/58N,pMW1
Application Note:
This recombinant protein can be used for WB. For research use only.
Background:
Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes. CAs form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons (or vice versa), a reversible reaction that occurs rather slowly in the absence of a catalyst. One of the functions of the enzyme in animals is to interconvert carbon dioxide and bicarbonate to maintain acid-base balance in blood and other tissues, and to help transport carbon dioxide out of tissues. The active site of most carbonic anhydrases contains a zinc ion. There are at least five distinct CA families (alpha, beta, γ, delta and epsilon).
Carbonic anhydrase 9 (CA9 / CAIX) is also known as Membrane antigen MN (MN), Renal cell carcinoma-associated antigen G250, which belongs to the alpha-carbonic anhydrase family. CA9 / CAIX with an optimal activity at pH 6.49. Reversible hydration of carbon dioxide. CA IX participates in pH regulation. CA9 may be involved in the control of cell proliferation and transformation. CA-IX appears to be a novel specific biomarker for a cervical neoplasia.
Background References:
- Badger MR, Price GD, 1994, Annu. Rev. Plant Physiol. Plant Mol. Bio. 45: 369?392.
- Temperini C., et al., 2007, Bioorg. Med. Chem. Lett. 17:2210-2215.
- Hilvo M., et al., 2008, J. Biol. Chem. 283:27799-27809.
- Alterio V., et al., 2009, Proc. Natl. Acad. Sci. U.S.A. 106:16233-16238.
Buffer:
50 mM Tris, 150 mM NaCl, pH7.5
Fusion Tag:
His Tag
NCBI Gene ID #:
768
NCBI Official Name:
carbonic anhydrase 9 precursor
NCBI Official Symbol:
Carbonic Anhydrase IX
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Predicted Molecular Weight:
30.9 kDa
Protein Accession #:
NP_001207.2
Purity:
>95% as determined by SDS-PAGE.
Endotoxin level is less than 1.0 EU per ug by the LAL method.
Endotoxin level is less than 1.0 EU per ug by the LAL method.
Recombinant Protein Sequence:
Thr32-Gly402
Source:
HEK293 cells
Swissprot #:
Q16790