TIGIT Recombinant Protein
| Code | Size | Price |
|---|
| PSI-97-026-0.05mg | 0.05mg | £729.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Mouse
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Lyophilized Protein should be stored at -20˚C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚C or -70˚C. Avoid repeated freeze-thaw cycles.
Documents
Further Information
Additional Names:
TIGIT,VSIG9,VSTM3
Application Note:
This recombinant protein can be used for E, WB. For research use only.
Background:
T-cell immunoreceptor with Ig and ITIM domains (TIGIT) is also known as V-set and immunoglobulin domain-containing protein 9 (VSIG9), V-set and transmembrane domain-containing protein 3 (VSTM3),which belongs to single-pass type I membrane protein containing an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT is expressed at low levels on peripheral memory and regulatory CD4+ T-cells and NK cells and is up-regulated following activation of these cells (at protein level). TIGIT binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells.
Background References:
- Yu X., et al., 2009, Nat. Immunol. 10:48-57.
- Stengel K.F., et al., 2012, Proc. Natl. Acad. Sci. U.S.A. 109:5399-5404.
- Lozano E., et al., 2012, J. Immunol. 188:3869-3875.
Biological Activity:
Measured by its binding ability in a functional ELISA. Immobilized Mouse TIGIT, Fc Tag at 5 ug/mL (100 uL/well) can bind Human Nectin-2, His Tag with a linear range of 2.5-20 ug/mL.
Buffer:
Tris?with?Glycine,?Arginine?and?NaCl,?pH7.5
Fusion Tag:
Fc Tag
NCBI Gene ID #:
100043314
NCBI Official Name:
T-cell immunoreceptor with Ig and ITIM domains precursor
NCBI Official Symbol:
TIGIT
NCBI Organism:
Mus musculus
Physical State:
Lyophilized
Predicted Molecular Weight:
40 kDa
Protein Accession #:
NP_001139797
Purity:
>95% as determined by SDS-PAGE.
Endotoxin level is less than 1.0 EU per ug by the LAL method.
Endotoxin level is less than 1.0 EU per ug by the LAL method.
Recombinant Protein Sequence:
Thr32-Gly402
Source:
HEK293 cells
Swissprot #:
NP_001139797