CTLA-4 Recombinant Protein
| Code | Size | Price |
|---|
| PSI-97-040-0.1mg | 0.1mg | £597.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Lyophilized Protein should be stored at -20˚C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚C or -70˚C. Avoid repeated freeze-thaw cycles.
Documents
Further Information
Additional Names:
CTLA4,CD152,CELIAC3,GRD4,GSE,ICOS,IDDM12
Application Note:
This recombinant protein can be used for E, WB. For research use only.
Background:
CTLA-4 (Cytotoxic T-Lymphocyte Antigen 4) is also known as CD152 (Cluster of differentiation 152), is a protein receptor that downregulates the immune system. CTLA4 is a member of the immunoglobulin superfamily, which is expressed on the surface of Helper T cells and transmits an inhibitory signal to T cells. The protein contains an extracellular V domain, a transmembrane domain, and a cytoplasmic tail. Alternate splice variants, encoding different isoforms. CTLA4 is similar to the T-cell co-stimulatory protein, CD28, and both molecules bind to CD80 and CD86, also called B7-1 and B7-2 respectively, on antigen-presenting cells. CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T cells and may be important to their function. Fusion proteins of CTLA4 and antibodies (CTLA4-Ig) have been used in clinical trials for rheumatoid arthritis.
Background References:
- Waterhouse P, et al., 1995, Science 270 (5238): 985?8.
- Magistrelli G, et al., 1999. Eur. J. Immunol. 29 (11): 3596?602.
- Rudd, CE. et al., 2009, Immunol. Rev.?229?(1): 12-26.
Biological Activity:
Measured by its binding ability in a functional ELISA. Immobilized Mouse B7-2, His Tag at 2 ug/mL (100 uL/well) can bind Rat CTLA-4, Fc Tag with a linear range of 0.2-6 ng/mL.
Buffer:
Tris?with?Glycine,?Arginine?and?NaCl,?pH7.5
Fusion Tag:
Fc Tag
NCBI Gene ID #:
63835
NCBI Official Name:
Cytotoxic T-lymphocyte associated protein-4 precursor
NCBI Official Symbol:
CTLA-4
NCBI Organism:
Rattus norvegicus
Physical State:
Lyophilized
Predicted Molecular Weight:
40.2 kDa
Protein Accession #:
Q62859
Purity:
>95% as determined by SDS-PAGE.
Endotoxin level is less than 1.0 EU per ug by the LAL method.
Endotoxin level is less than 1.0 EU per ug by the LAL method.
Recombinant Protein Sequence:
Thr32-Gly402
Source:
HEK293 cells
Swissprot #:
N/A