VISTA Antibody [4C4]

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ProSci
Product Code: PSI-RF16071
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-RF16071-0.02mg0.02mg£150.00
Quantity:
PSI-RF16071-0.1mg0.1mg£515.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: 4C4
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Flow Cytometry
  • Immunocytochemistry (ICC)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Shipping:
blue ice
Storage:
VISTA antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of VISTA in overexpressing HEK293 cells with VISTA antibody at (A) 0.25 (B) 0.5 and (C) 1 μg/ml
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Immunocytochemistry of VISTA in transfected HEK293 cells with VISTA antibody at 1 μg/mL. Lower left: Immunocytochemistry in transfected HEK293 cells with control mouse IgG antibody at 1 μg/mL.
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Immunofluorescence of VISTA in transfected HEK293 cells with VISTA antibody at 2 μg/mL. <br><br>Green: VISTA Antibody [4C4] (RF16071) <br> Blue: DAPI staining
4 / 8
Immunofluorescence of VISTA in human lymphoma tissue with VISTA antibody at 5 μg/mL. <br><br>Red: VISTA Antibody [4C4] (RF16071) <br> Blue: DAPI staining
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Immunofluorescence of VISTA in human spleen tissue with VISTA antibody at 10 μg/mL. <br><br>Red: VISTA Antibody [4C4] (RF16071) <br> Blue: DAPI staining
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Immunohistochemistry of VISTA in human lymphoma tissue with VISTA antibody at 2 μg/mL.
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Flow cytometry analysis of VISTA overexpressing HEK293 cells using VISTA antibody and control mouse IgG antibody at 10 μg/ml. Blue: Untransfected HEK293 cells. Yellow: VISTA overexpressing HEK293 cells.
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Titration curve analysis of VISTA antibody to detect recombinant VISTA in ELISA at decreasing concentrations.
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Western blot analysis of VISTA in overexpressing HEK293 cells with VISTA antibody at (A) 0.25 (B) 0.5 and (C) 1 μg/ml
Immunocytochemistry of VISTA in transfected HEK293 cells with VISTA antibody at 1 μg/mL. Lower left: Immunocytochemistry in transfected HEK293 cells with control mouse IgG antibody at 1 μg/mL.
Immunofluorescence of VISTA in transfected HEK293 cells with VISTA antibody at 2 μg/mL. <br><br>Green: VISTA Antibody [4C4] (RF16071) <br> Blue: DAPI staining
Immunofluorescence of VISTA in human lymphoma tissue with VISTA antibody at 5 μg/mL. <br><br>Red: VISTA Antibody [4C4] (RF16071) <br> Blue: DAPI staining
Immunofluorescence of VISTA in human spleen tissue with VISTA antibody at 10 μg/mL. <br><br>Red: VISTA Antibody [4C4] (RF16071) <br> Blue: DAPI staining
Immunohistochemistry of VISTA in human lymphoma tissue with VISTA antibody at 2 μg/mL.
Flow cytometry analysis of VISTA overexpressing HEK293 cells using VISTA antibody and control mouse IgG antibody at 10 μg/ml. Blue: Untransfected HEK293 cells. Yellow: VISTA overexpressing HEK293 cells.
Titration curve analysis of VISTA antibody to detect recombinant VISTA in ELISA at decreasing concentrations.

Documents

Further Information

Additional Names:
VISTA Antibody: VISTA molecule, VSIR, B7-H5, B7H5, GI24, PP2135, SISP1, DD1alpha, VISTA, C10orf54, chromosome 10 open reading frame 54, PD-1H, V-set immunoregulatory receptor, V-Type Immunoglobulin Domain-Containing Suppressor Of T-Cell Activation, Chromosome 10 Open Reading Frame 54
Application Note:
VISTA antibody can be used for detection of VISTA by Western blot at 0.25 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL and immunocytochemistry starting at 1 μg/mL. For immunofluorescence start at 2 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples; Immunofluorescence in human samples and Flow Cytometry in mouse samples. All other applications and species not yet tested.
Background:
VISTA Antibody: VISTA/B7-H5/platelet receptor Gi24 is a single-pass type I membrane protein located at the cell surface. It is an immunoregulatory receptor which can inhibit T-cell response and may promote differentiation of embryonic stem cells, by inhibiting the BMP4 signaling pathway. The protein can be cleaved by MMP14, and stimulate MMP14-mediated MMP2 activation.
Background References:
  • Mayya V., et al . Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions. 2009, Sci. Signal. 2:RA46-RA46.
  • Sakr M.A., et al.,GI24 enhances tumor invasiveness by regulating cell surface membrane-type 1 matrix metalloproteinase. 2010, Cancer Sci. 101:2368-2374.
Buffer:
VISTA Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
VISTA antibody was raised against the extracellular domain of human VISTA.
NCBI Gene ID #:
64115
NCBI Official Name:
VISTA molecule
NCBI Official Symbol:
VSIR
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 31 kDa

Observed: 47 kDa
Protein Accession #:
NP_071436
Protein GI Number:
64115
Purification:
VISTA Antibody is supplied as protein A purified IgG1.
Research Area:
Immunology
Swissprot #:
Q9H7M9
User NOte:
Optimal dilutions for each application to be determined by the researcher.