Collagen Type II chicken
| Code | Size | Price |
|---|
| CO20231-0.1 | 0.1 ml | £411.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Chicken (Gallus)
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Radioimmunoassay (RIA)
Storage:
-20°C
Documents
Further Information
Applications Description:
IHC(P), IFA, ELISA, RIA
Background:
Type II collagen is an alpha1(II)-trimer, which forms 67 nm cross-banded fibrils. Typically it can be observed in cartilage and various tumours. Collagens consist of a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule consists of a triple-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Chicken collagen type II 100%, no crossreactivity with chicken collagen type I and III, no crossreactivity with human, mouse and rat collagen type II..
Caution:
*These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.
Concentration:
app. 1 mg/ml
Immunogen:
Purified collagen type II from fetal chicken cartilage
Incubation Time:
IHC(P) 60 min at RT or 2-8°C over night
Positive Control:
Chicken cartilage
Pre-Treatment:
After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg e.g. Art. No. HYA02-50) in TBS 15 min at 37°C. Thereafter non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.
Product:
affinity purified antibody lyophilized from phosphate buffered solution; no BSA and preservative added!
Purification Method:
affinity purified antibody lyophilized from phosphate buffered solution; no BSA and preservative added!
Secondary Reagents:
Anti-rabbit IgG-conjugates, e.g. anti-rabbit IgG:FITC (Art. No. FI-1000) or anti-rabbit IgG:DyLight488 (Art. No. DI-1488).
Species Reactivity:
Chicken
UniProt:
P02460 (CO2A1_CHICK)
Working Concentration:
(purified, lyophilized) IFA 1:40, IHC(P) 1:200 - 1:600
References
1. Tacchetti C., Quarto R., Nitsch L., Hartmann D.J., Cancedda R. (1987) In vitro morphogenesis of chick embryo hypertrophic cartilage. J. Cell. Biol. 105, 999-1006.
2. Mallein-Gerin F., Ruggiero F., Garrone R. (1990) Proteoglycan core protein and type II collagen gene expressions are not correlated with cell shape changes during low density chondrocyte cultures. Differentiation 43, 204-211.
3. Anselme K., Lanel B., Gentil C., Hardouin P., Marie P.J., Sigot-Luizard M.F. (1994) Bone organotypic culture method: a model for cytocompatibility testing of biomaterials. Cells and Materials 4, 113-123.
4. Rousseau J.C., Farjanel J., Boutillon M.M., Hartmann D.J., van der Rest M., Moradi-Ameli M. (1996) Processing of Type XI Collagen. J. Biol. Chem. 271, 23743-23748.
2. Mallein-Gerin F., Ruggiero F., Garrone R. (1990) Proteoglycan core protein and type II collagen gene expressions are not correlated with cell shape changes during low density chondrocyte cultures. Differentiation 43, 204-211.
3. Anselme K., Lanel B., Gentil C., Hardouin P., Marie P.J., Sigot-Luizard M.F. (1994) Bone organotypic culture method: a model for cytocompatibility testing of biomaterials. Cells and Materials 4, 113-123.
4. Rousseau J.C., Farjanel J., Boutillon M.M., Hartmann D.J., van der Rest M., Moradi-Ameli M. (1996) Processing of Type XI Collagen. J. Biol. Chem. 271, 23743-23748.