Collagen Type I chicken
| Code | Size | Price |
|---|
| CO20131-0.1 | 0.1 ml | £480.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Bovine (Cattle)
- Chicken (Gallus)
- Human
- Mouse
- Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunoblotting
- Immunofluorescence (IF)
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Radioimmunoassay (RIA)
- Western Blot (WB)
Storage:
-20°C
Documents
Further Information
Applications Description:
IHC(P), IFA, ELISA, RIA, IB/WB
Background:
Type I Collagen usually exists as a heterotrimer formed by alpha 1(I) and alpha 2(I) chains and is found in bone, cornea, skin and tendon. In foetal tissues also homotrimers of alpha-1(I) are found, but they are not constituents of normal adult tissues. Collagens consist of a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule consists of a triple-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Chicken collagen type I 100%, chicken collagen type II, III and V <0.1%; chicken fibronectin <0.1% RIA at 1:1000 dilution..
Caution:
*These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.
Concentration:
app. 1 mg/ml
Immunogen:
Purified collagen type I from chicken skin
Incubation Time:
IHC(P) 60 min at RT or 2-8°C over night
Positive Control:
Chicken skin
Pre-Treatment:
After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg e.g. Art. No. HYA02-50) in TBS 15 min at 37°C. Thereafter non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.
Product:
affinity purified antibody lyophilized from phosphate buffered solution; no BSA and preservative added!
Purification Method:
affinity purified antibody lyophilized from phosphate buffered solution; no BSA and preservative added!
Secondary Reagents:
Anti-rabbit IgG-conjugates, e.g. anti-rabbit IgG:FITC (Art. No. FI-1000) or anti-rabbit IgG:DyLight488 (Art. No. DI-1488).
Species Reactivity:
Chicken, collagen type I human, mouse, rat, cattle <0.1%
UniProt:
P02457
Working Concentration:
(purified, lyophilized) IFA ? 1:80, IHC(P) ? 1:1000, ELISA ? 1:200 (OD ? 500)
References
1. Mauger A., Demarchez M., Herbage D., Grimaud J.A., Druguet M., Hartmann D., Sengel P. (1982) Immunofluorescent localization of collagen types I and III, and of fibronectin during feather morphogenesis in the chick embryo. Dev. Biol. 94, 93-105.
2. Tacchetti C., Quarto R., Nitsch L., Hartmann D.J., Cancedda R. (1987) In vitro morphogenesis of chick embryo hypertrophic cartilage. J. Cell. Biol. 105, 999-1006.
3. Robert J., Hartmann D.J., Sengel P. (1989) Production of fibronectin and collagen types I and III by chick embryo dermal cells cultured on extracellular matrix substrates. Int. J. Dev. Biol. 33, 267-275.
4. Farjanel J., Hartmann D.J., Guidet B., Luquel L., Offenstadt G. (1993) Four markers of collagen metabolism as possible indicators of disease in the adult respiratory distress syndrome. Am. Rev. Respir. DIs. 147, 1091-1099.
2. Tacchetti C., Quarto R., Nitsch L., Hartmann D.J., Cancedda R. (1987) In vitro morphogenesis of chick embryo hypertrophic cartilage. J. Cell. Biol. 105, 999-1006.
3. Robert J., Hartmann D.J., Sengel P. (1989) Production of fibronectin and collagen types I and III by chick embryo dermal cells cultured on extracellular matrix substrates. Int. J. Dev. Biol. 33, 267-275.
4. Farjanel J., Hartmann D.J., Guidet B., Luquel L., Offenstadt G. (1993) Four markers of collagen metabolism as possible indicators of disease in the adult respiratory distress syndrome. Am. Rev. Respir. DIs. 147, 1091-1099.