ACHE Antibody

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ProSci
Product Code: PSI-45-193
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-193-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Shipping:
blue ice
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-193 (1ug/ml) staining of HepG2 (A), Daudi (B) and (0.3ug/ml) HeLa (C) and Jurkat (D) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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45-193 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml). IgG control: Unimmunized goat IgG (black line) fol
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45-193 (1ug/ml) staining of HepG2 (A), Daudi (B) and (0.3ug/ml) HeLa (C) and Jurkat (D) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
45-193 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml). IgG control: Unimmunized goat IgG (black line) fol

Documents

Further Information

Additional Names:
ACHE, YT, acetylcholinesterase (YT blood group), ARACHE, N-ACHE, OTTHUMP00000211347, OTTHUMP00000211349, OTTHUMP00000211356, acetylcholinesterase, apoptosis-related acetylcholinesterase
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 70kDa band observed in HepG2 and Jurkat cell lysates and approx. 65-70kDa in Daudi and HeLa cell lysates (calculated MW of 67.8kDa according to NP_000656.1). Recommended concentration: 0.3-1?g/ml. Primary incubation 1 hour at room temperature.Immunofluorescence: Strong expression of the protein seen in the cytoplasm and nuclei of U2OS cells. Recommended concentration: 10ug/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Background References:
  • Cottingham MG, Voskuil JL, Vaux DJ. The intact human acetylcholinesterase C-terminal oligomerization domain is alpha-helical in situ and in isolation, but a shorter fragment forms beta-sheet-rich amyloid fibrils and protofibrillar oligomers. Biochemistry. 2003 Sep 16;42(36):10863-73.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse
Immunogen:
The immunogen for this antibody is: QFDHYSKQDRCSDL
NCBI Gene ID #:
43
NCBI Official Name:
acetylcholinesterase (Yt blood group)
NCBI Official Symbol:
ACHE
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_000656.1, NP_001269378.1
Protein GI Number:
4557239
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to recognise isoform NP_000656.1 only (the ubiquitously expressed, hydrophillic form).
Swissprot #:
P22303