Collagen Type IV human

IHC(P), IFA, ELISA, RIA, IB/WB

Type IV Collagen is a non-fibrillary network of different alpha-chains: alpha 1(IV) to-alpha 6(IV). It is typically found in basal membranes of different organs (e.g. skin, lens, lung, kidney). Collagens consist of a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule consists of a triple-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Human collagen type IV 100%, human collagen types I, II, III, and V <0.1%, human fibronectin and laminin < 0.1% (RIA at 1:10000 dilution).

*These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.

app. 1 mg/ml

Purified collagen type IV from human placenta

IHC(P) 60 min at RT or 2-8°C over night

Human skin, placenta or lens capsule

After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg e.g. Art. No. HYA02-50) in TBS 15 min at 37°C. Thereafter non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.

0.1 ml affinity purified antibody prod. in nude mice; lyophilized from phosphate buffered solution; no BSA and preservative added!

0.1 ml affinity purified antibody prod. in nude mice; lyophilized from phosphate buffered solution; no BSA and preservative added!

Anti-mouse IgG-conjugates, e.g. Anti-mouse IgG:FITC (Art. No. FI-2000) or anti-mouse IgG:DyLight488 (Art. No. DI-2488).

Mouse monoclonal antibody obtained from fusion of splenocytes of animals immunized with purified collagen type IV from human placenta.

Human, no cross-reactivity with bovine, mouse, rat and porcine collagens type IV

P02462 (CO4A1_HUMAN) ; P08572 (CO4A2_HUMAN); P29400 (CO4A5_HUMAN);

(purified, lyophilized) IFA ? 1:320, IHC(P) ? 1:4000, ELISA ? 1:4000 (OD ? 500)