CD5 human

IHC(C, P), FACS

The antibody to CD5 is used for the classification of lymphoid neoplasm. CD5 is exprimed by the majority of T-cell lymphoma and is also found on B-cells in some cases of chronic lymphatic leukaemia and centrocytic lymphoma. Normally it is not found in other forms of lymphoid neoplasm e.g. follicular lymphoma, Hairy Cell Lymphoma and Large Cell Lymphoma. CD5 is a lymphocyte surface molecule with a molecular weight of 67 kDa. It is exprimed by 95% of thymocytes and app. 72% of peripheral blood lymphocytes. In lymph nodes the antigen is detected in the T-cell region mainly. CD5 is over-expressed in leukaemia and T-cell lymphoma and may also be detected in a subpopulation of activated B-cells. Human CD5 molecule.

*These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.
** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!

Synthetic Protein, corresponding to a sequence of human CD5 molecule

60 min at RT

Tonsil

Paraffin sections are pre-treated with 1 mM EDTA (pH 8.0) in an autoclave (2 min under full pressure, cooling 15-20 min.)

Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 ?l working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art.No. PU002.

Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 ?l working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art.No. PU002.

We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005).

Human

P06127

(liquid conc.) 1:10-1:25