HEA125 Epithelium-specific Glycoprotein Egp34 EpCAM (CD326)
| Code | Size | Price |
|---|
| HE100-2 | 100 ug | £578.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: HEA125
Regulatory Status: RUO
Target Species: Human
Applications:
- Fluorescence-activated cell sorting (FACS)
- Immunohistochemistry- Frozen Section (IHC-F)
- Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
2-8°C
Documents
Further Information
Applications Description:
IHC(C, P), FACS
Background:
The antibody HEA 125 reacts intensely with all carcinoma derived from the intestinal tract, stomach, pancreas, liver, lung, breast, ovary, thyroid gland, kidney, bladder, prostate and metastases thereof. Stratified squamous epithelia usually stain less intensely then adenocarcinoma. The antibody is suitable for differentiation between carcinoma an non-carcinoma. Keratinizing areas of a tumour mass usually remain unstained as sarcoma, lymphoma, melanoma and neurogenic tumours do. Epithelial cells express a number of characteristic proteins and glycoproteins like cytokeratins, epithelial membrane antigen and epithelium-specific glycoprotein Egp34 (HEA125). The monoclonal antibody HEA125 which has been derived from immunisation of mice with a colon carcinoma cell line shows a broad reactivity pattern. It is labelling most human epithelial cells and epithelium derived tumours. It doesn't react with epidermal keratinocytes, parietal cells, hepatocytes, thymus cortical epithelium, myoepithelia, and non-epithelial tissues. 34 kDa Epithelium-specific membrane glycoprotein HEA 125.
Caution:
*These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.
** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
Concentration:
100 ?g/ml
Immunogen:
Living human colon carcinoma cell line HT-29
Incubation Time:
60 min at RT
Positive Control:
Appendix
Pre-Treatment:
Pre-treatment with pronase 0.1% or unmasking fluid C (Art. No. DE000)
Product:
Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 ?l working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art .No. PU002.
Purification Method:
Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 ?l working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art .No. PU002.
Secondary Reagents:
We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005).
Species Reactivity:
Human
UniProt:
P16422 (EPCAM_HUMAN)
Working Concentration:
(liquid conc.) 1:20-1:100
References
1. Moldenhauer G., Momburg F., M?ller P., Schwartz, and H?mmerling G.J. (1987) Epithelium-specific surface glycoprotein of Mr 34,000 is a widely distributed human carcinoma marker. Br. J. Cancer 56; 714-721.
2. Momburg F., Moldenhauer G., H?mmerling G.J., and M?ller F. (1987) Immunohistochemical study of the expression of a Mr 34,000 human epithelium-specific surface glycoprotein in normal and malignant tissues. Cancer Research 47; 2883-2891.
3. Simon B., Podolsky, D.K., Moldenhauer G., Isselbacher K.J., Gattoni-Celli, S., and Brand S.J. (1990) Epithelial glykoprotein is a member of a family of epithelial cell surface antigens homologous to nidogen, a matrix adhesion protein. Proc. Natl., Acad. Sci. USA 87; 2755 ff.
4. Kemmner W., Moldenhauer G., Schlag P., and Brossmer R. (1992) Separation of tumor cells from a suspension of dissociated human colorectal carcionoma tissue by means of monoclonal antibody-coated magnetic beads. J. Immunol. Methods 147; 197-200.
5. Winter , M. J. et al. (2003) The epithelial cell adhesion molecule (Ep - CAM) as a morphoregulatory molecule is a tool in surgical pathology. Am. J. Pathol. 163 : 2139 - 2148.
2. Momburg F., Moldenhauer G., H?mmerling G.J., and M?ller F. (1987) Immunohistochemical study of the expression of a Mr 34,000 human epithelium-specific surface glycoprotein in normal and malignant tissues. Cancer Research 47; 2883-2891.
3. Simon B., Podolsky, D.K., Moldenhauer G., Isselbacher K.J., Gattoni-Celli, S., and Brand S.J. (1990) Epithelial glykoprotein is a member of a family of epithelial cell surface antigens homologous to nidogen, a matrix adhesion protein. Proc. Natl., Acad. Sci. USA 87; 2755 ff.
4. Kemmner W., Moldenhauer G., Schlag P., and Brossmer R. (1992) Separation of tumor cells from a suspension of dissociated human colorectal carcionoma tissue by means of monoclonal antibody-coated magnetic beads. J. Immunol. Methods 147; 197-200.
5. Winter , M. J. et al. (2003) The epithelial cell adhesion molecule (Ep - CAM) as a morphoregulatory molecule is a tool in surgical pathology. Am. J. Pathol. 163 : 2139 - 2148.