Ki-67 Cell Cycle Marker (MIB-1)
| Code | Size | Price |
|---|
| KI500 | 5 ml | £409.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: MIB-1
Regulatory Status: RUO
Target Species: Human
Applications:
- Immunohistochemistry- Frozen Section (IHC-F)
- Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
2-8°C
Documents
Further Information
Applications Description:
IHC(C, P)
Background:
In retrospective studies the intensity of staining and the frequency of Ki-67 expression in cell nuclei was interpreted as a prognostic factor for the malignancy of tumours (Gerdes et al., 1987). Ki-67 Protein is a nuclear protein, which is ubiquitous present during cell division, that means in all phases of mitosis (S1, G1, G2 and M phase). The protein is strongly exprimed in proliferating tissues e.g. embryonal tissue and rapidly growing tumours. Ki-67 nuclear protein.
Caution:
*These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.
** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
Immunogen:
Human nuclear Ki-67 antigen
Incubation Time:
60 min at RT
Positive Control:
Breast or colon carcinoma
Pre-Treatment:
Unmasking fluid G (Art. No. DE007) or C (Art. No. DE000) at 90-100°C
Product:
Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 50 immunohistochemical tests (100 ?l working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art. No. PU002, if further dilution is required.
Purification Method:
Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 50 immunohistochemical tests (100 ?l working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art. No. PU002, if further dilution is required.
Secondary Reagents:
We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005).
Species Reactivity:
Human
UniProt:
P46013
Working Concentration:
(RTU) neat
References
1. Gerdes J., Schwab U., Lemke H., and Stein H. (1983) Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation. Int. J. Cancer 31; 13-20.
2. Gerdes J., Stein H., Pileri S., Rivano M.T., Gobbi M., Ralfkiaer E., Nielsen K.M., Pallesen G., Bartels H., Palestro G., and Delsol G. (1987) Prognostic relevance of the tumour-cell growth fraction in malignant non-Hodgkin's lymphomas. Letter to the editor: Lancet ii, 448-449.
3. Gerdes J., Becker M.H.G., Key G., and Cattoretti G. (1992) Immunohistochemical detection of tumour growth fraction (Ki-67 antigen) in formalin-fixed and routinely processed tissues. J. Pathol., 168; 85-86.
4. Kubbutat M.H.G., Cattoretti G., Gerdes J., Key G., (1994) Comparison of monoclonal antibodies PC10 and MIB-1 on microwave-processed paraffin sections. Cell Proliferation 27; 553-559.
2. Gerdes J., Stein H., Pileri S., Rivano M.T., Gobbi M., Ralfkiaer E., Nielsen K.M., Pallesen G., Bartels H., Palestro G., and Delsol G. (1987) Prognostic relevance of the tumour-cell growth fraction in malignant non-Hodgkin's lymphomas. Letter to the editor: Lancet ii, 448-449.
3. Gerdes J., Becker M.H.G., Key G., and Cattoretti G. (1992) Immunohistochemical detection of tumour growth fraction (Ki-67 antigen) in formalin-fixed and routinely processed tissues. J. Pathol., 168; 85-86.
4. Kubbutat M.H.G., Cattoretti G., Gerdes J., Key G., (1994) Comparison of monoclonal antibodies PC10 and MIB-1 on microwave-processed paraffin sections. Cell Proliferation 27; 553-559.