Cytokeratin 7
| Code | Size | Price |
|---|
| CK507 | 5 ml | £300.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: OV-TL12/30
Regulatory Status: RUO
Target Species: Human
Applications:
- Immunohistochemistry- Frozen Section (IHC-F)
- Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
2-8°C
Documents
Further Information
Applications Description:
IHC(C, P)
Background:
Cytokeratin 7 is a basic filament protein, which is exprimed by many epithelial cells, especially those of the ductal and glandular types. The antibody doesn't react with squamous epithelia but with transitional cell epithelia of the urinary tract. Epithelia of the lung and breast are positive while colon and prostate remain unstained. The antibody is helpful for the differentiation of colon and ovarian cancer cells in ascites. Cytokeratins are a group of water soluble filament proteins, which are components of the cytoskeleton of the epidermis and other epithelial cells. Gel-electrophoretic investigation revealed 20 different Cytokeratins, characterised by different molecular sizes and pI-values. They can be subdivided into basic and acid subfamilies. The nomenclature according to Moll et al. (1982) is most commonly used. Basic Cytokeratin, 54 kD, CK 7 (Moll).
Caution:
*These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.
** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
Immunogen:
Ovarian carcinoma cell line OTN 11
Incubation Time:
60 min at RT
Positive Control:
Ovarian carcinoma
Pre-Treatment:
Improvement of staining with Unmasking Fluid G (Art. No. DE007) or Unmasking Fluid C (Art. No. DE000)
Product:
Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 50 immunohistochemical tests (100 ?l working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art. No. PU002, if further dilution is required.
Purification Method:
Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 50 immunohistochemical tests (100 ?l working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art. No. PU002, if further dilution is required.
Secondary Reagents:
We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005).
Species Reactivity:
Human
UniProt:
P08729
Working Concentration:
(RTU) neat
References
1. Moll R., Franke W.W., Schiller D.L., Geiger B., and Krepler R. (1982) The Catalog of Human Cytokeratins: Patterns of Expression in Normal Epithelia, Tumors and Cultured Cells. Cell 31; 11 ff.
2. Sun T.-T. Tseng S.C.G., Huang A.J.W., Cooper D., Lynch M.H., Weiss R., Eichner R., and Schermer (1985) Monoclonal antibody studies of keratin expression: A review. In: Intermediate Filaments , Wang E. et al. eds. N.Y. Acad. Sci. 455, pp 307 ff.
3. Ramaekers F., Huysmans A., Schaart G., Moesker O., Vooijs P. (1987) Tissue distribution of keratin 7 as monitored by a monoclonal antibody. Exp. Cell Res. 170; 235-249.
4. Van de Molenkraft F.J.J.M., van Niekerk C.C., Jap P.H.K., Poels L.G. (1993) OV-LT 12/13 (keratin 7 antibody) is a marker of glandular differentiation in lung cancer. Histopathology 22; 35-38.
2. Sun T.-T. Tseng S.C.G., Huang A.J.W., Cooper D., Lynch M.H., Weiss R., Eichner R., and Schermer (1985) Monoclonal antibody studies of keratin expression: A review. In: Intermediate Filaments , Wang E. et al. eds. N.Y. Acad. Sci. 455, pp 307 ff.
3. Ramaekers F., Huysmans A., Schaart G., Moesker O., Vooijs P. (1987) Tissue distribution of keratin 7 as monitored by a monoclonal antibody. Exp. Cell Res. 170; 235-249.
4. Van de Molenkraft F.J.J.M., van Niekerk C.C., Jap P.H.K., Poels L.G. (1993) OV-LT 12/13 (keratin 7 antibody) is a marker of glandular differentiation in lung cancer. Histopathology 22; 35-38.