Melanoma HMB45

IHC(P)

The antibody HMB45 is used for the discrimination of Melanoma from normal Melanocytes. Melanoic Schwannoma Clear Cell Sarcoma will also be stained. Negative are tumours of epithelial, lymphoid, glial and mesenchymal origin. Melanocytes are pigmented, melanin containing cells being located mainly in the epidermis. They protect tissues from UVB irradiation of the sun. The antibody HMB45 reacts with fetal and neonatal Melanocytes, with junctional and blue naevus cells, but not with intradermal naevi and adult melanocytes. Neuraminidase-sensitive glycoconjugate on the surface of human melanosomes.

*These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.
** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!

Extract from lymph node metastases of melanoma

60 min at RT

Melanoma

Pre-treatment with pronase 0,1% or unmasking fluid G (Art. No. DE007)

Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 50 immunohistochemical tests (100 ?l working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art. No. PU002, if further dilution is required.

Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 50 immunohistochemical tests (100 ?l working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art. No. PU002, if further dilution is required.

We recommend the use of BIOLOGO'S Universal Staining System AEC (Art.-No. AE005), but also other sensitive staining systems are applicable (red staining usually is preferred).

Human

P40967

(RTU) neat