PIN-Cocktail 2 --- P504S / p63 / HMW Cytokeratin

IHC(C, P)

The combination of AMACR (P504S), p63, and HMW CK may be extremely useful for diagnosing PIN and small focus adenocarcinoma, especially in difficult cases and cases with limited tissues. The combination of p63 and HMW CK antibodies provides a save method for the detection of basal cells, while AMACR (P504S) labels only carcinoma cells and PIN lesions. P504S (AMACR, Alpha-methylacyl-CoA racemase) is an essential enzyme in the b-oxidation of branched-chain fatty acids. High expression of AMACR protein is found in prostate adenocarcinoma but not in benign prostate tissue by immunohistochemical staining in paraffin-embedded tissue. The expression of AMACR is also detected in prostate premalignant lesions, such as prostate intraepithelial neoplasia (PIN). The p63 protein, a homologue of the tumor-suppressor p53, is highly expressed in the nuclei of basal or progenitor layer of many epithelial tissues. p63 is detected in prostate basal cells in normal prostate glands and PIN. Other markers for basal cells are high molecular cytokeratins (CK 1, 5, 10 and 14), which can be labeled with HMW CK clone 34?E12. In prostate adenocarcinoma basal cells disappear and staining with both p63 and HMW CK fails. Thus the combination of p63 and HMW CK is a useful tool as differential markers for benign prostate glands and adenocarcinoma (negative marker). 1. Human AMACR (P504S) protein, Alpha-Methylacyl CoA Racemase, 2. human p63 nuclear protein, and 3. high molecular weight cytokeratins (56-57 and 66-68 kDa).

*These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.
** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!

1. Human recombinant AMACR (P504S) protein, whole sequence (for rabbit monoclonal antibody 13H4); 2. Recombinant human p63 protein aa 1-205 (for mouse monoclonal antibody 4A4); 3. Cytokeratin extract of human stratum corneum (for mouse monoclonal antibody 34?E12)

60 min at RT

Prostatic intraepithelial neoplasia

Use formalin-fixed and paraffin-embedded sections; Retrieval conditions: Unmasking fluid T, TEC buffer (Tris/EDTA/Citrate) pH 8 (Art. No. DE005) in a pressure cooker at 100°C 20-40 minutes

Affinity purified antibodies in PBS, BSA, sodium azide (0.09%)**. Use antibody dilution buffer (e.g. Art. No. PU002) containing sufficient protein and preservative.

Affinity purified antibodies in PBS, BSA, sodium azide (0.09%)**. Use antibody dilution buffer (e.g. Art. No. PU002) containing sufficient protein and preservative.

Rabbits were immunized with human recombinant AMACR (P504S) protein, whole sequence (for rabbit monoclonal antibody 13H4); Mice were immunized with recombinant human p63 protein aa 1-205 (for mouse monoclonal antibody 4A4) or a cytokeratin extract of human stratum corneum (for mouse monoclonal antibody 34?E12).

Human

(RTU) neat