Pan-Cytokeratin 2 Clones (AE1/AE3)
| Code | Size | Price |
|---|
| CK102 | 1 ml | £445.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: AE1/AE3
Regulatory Status: RUO
Target Species:
- Bovine (Cattle)
- Chicken (Gallus)
- Human
- Mouse
- Rabbit
- Rat
Applications:
- Immunohistochemistry- Frozen Section (IHC-F)
- Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
2-8°C
Documents
Further Information
Applications Description:
IHC(C, P)
Background:
Pan-Cytokeratin (AE1/AE3) contains two different clones, which label the majority of normal epithelia and carcinoma. Cytokeratins are a group of water soluble filament proteins, which are components of the cytoskeleton of the epidermis and other epithelial cells. Gelelectrophoretic investigation revealed 20 different Cytokeratins, characterised by different molecular sizes and pI-values. They can be subdivided into basic and acid subfamilies. The nomenclature according to Moll et al. (1982) is most commonly used. Basic cytokeratins (52-67 kD) and acid cytokeratins (40, 48, 50, 50', 56,5 kD) CK 1-8, 10, 14-16 and 19 according to the nomenclature by Moll (1982) .
Caution:
*These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.
** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
Concentration:
70 ?g/ml
Immunogen:
Human epithelial keratins
Incubation Time:
60 min at RT
Positive Control:
Appendix
Pre-Treatment:
Paraffin sections may be pre-treated with 1 % protease solution (Unmasking fluid P, Art.-No. DE110) for 15 min at RT, improvement of staining may be achieved with Unmasking Fluid G (Art. No. DE007) or Unmasking Fluid C (Art. No. DE000).
Product:
Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 200 immunohistochemical tests (100 ?l working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art .No. PU002.
Purification Method:
Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 200 immunohistochemical tests (100 ?l working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art .No. PU002.
Secondary Reagents:
We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005).
Species Reactivity:
Human, chicken, cattle, rabbit, mouse, rat
Working Concentration:
(liquid conc.) 1:20 - 1:50
References
1. Moll R., Franke W.W., Schiller D.L., Geiger B., and Krepler R. (1982) The Catalog of Human Cytokeratins: Patterns of Expression in Normal Epithelia, Tumors and Cultured Cells. Cell 31; 11 ff.
2. Sun T.-T. Tseng S.C.G., Huang A.J.W., Cooper D., Lynch M.H., Weiss R., Eichner R., and Schermer (1985) Monoclonal antibody studies of keratin expression: A review. In: Intermediate Filaments , Wang E. et al. eds. N.Y. Acad. Sci. 455, pp 307 ff.
2. Sun T.-T. Tseng S.C.G., Huang A.J.W., Cooper D., Lynch M.H., Weiss R., Eichner R., and Schermer (1985) Monoclonal antibody studies of keratin expression: A review. In: Intermediate Filaments , Wang E. et al. eds. N.Y. Acad. Sci. 455, pp 307 ff.