CD68 (KP1) Macrophages human
| Code | Size | Price |
|---|
| CD68KP1-0.5 | 0.5 ml | £515.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG1 k
Antibody Clonality: Monoclonal
Antibody Clone: KP1
Regulatory Status: RUO
Target Species:
- Feline (cat)
- Human
- Monkey
- Rat
Applications:
- Immunohistochemistry- Frozen Section (IHC-F)
- Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
2-8°C
Documents
Further Information
Applications Description:
IHC(C, P)
Background:
The antibody KP1 is well suited for the detection of tissue macrophages including Kupffer cells and spleen macrophages. CD68 is expressed on macrophages and monocytes. KP-1 is important for identifying macrophages in tissue sections. It stains macrophages in a wide variety of human tissues, including Kupffer cells and macrophages in the red pulp of the spleen, in lamina propria of the gut, in lung alveoli, and in bone marrow. KP-1 reacts with myeloid precursors and peripheral blood granulocytes. It also reacts with plasmacytoid T cells which are supposed to be of monocyte/macrophage origin. It shows strong granular cytoplasmic staining of chronic and acute myeloid leukemia and also reacts with rare cases of true histiocytic neoplasia. Tumors of lymphoid origin are usually not stained. CD68 transmembran glycoprotein MW 110 kDa, localised in macrophages.
Caution:
*These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.
** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
Immunogen:
Subcellular fraction of human alveolar macrophages
Incubation Time:
60 min at RT
Positive Control:
Tonsil, spleen, lymph node
Pre-Treatment:
Unmasking with Pronase (Art. No. DE110)
Product:
Purified antibody with 0.2% BSA and 15 mM sodium azide as preservative
Purification Method:
Purified antibody with 0.2% BSA and 15 mM sodium azide as preservative
Secondary Reagents:
We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005).
Species Reactivity:
Human, monkey, cat and rat. Does not react with pig, dog and chicken
Working Concentration:
(liquid conc.) 1:100-1:200
References
1. Fukuda M. (1991) Lysosomal membrane glycoproteins. Structure, biosynthesis, and intracellular trafficing. J. Biol. Chem. 266; 21327-21330.
2. Holness C.I. and Simmons D.L. (1993) Molecular cloning of CD68, a human macrophage marker related to lysosomal glycoproteins. Blood 81; 1607-1613.
3. Pulford KAF, Rigney EM, Micklem KJ, Jones M, Stross WP, Gatter KC, et al. (1989) KP1: a new monoclonal antibody that detects a monocyte/macrophage associated antigen in routinely processed tissue sections. J Clin Pathol;42:414-21.
4. Warnke RA, Pulford KAF, Pallesen G, Ralfkiaer E, Brown DC, Gatter KC, et al. (1989) Diagnosis of myelomonocytic and macrophage neoplasms in routinely processed tissue biopsies with monoclonal antibody KP1. Am J Pathol;135:1089-95.
2. Holness C.I. and Simmons D.L. (1993) Molecular cloning of CD68, a human macrophage marker related to lysosomal glycoproteins. Blood 81; 1607-1613.
3. Pulford KAF, Rigney EM, Micklem KJ, Jones M, Stross WP, Gatter KC, et al. (1989) KP1: a new monoclonal antibody that detects a monocyte/macrophage associated antigen in routinely processed tissue sections. J Clin Pathol;42:414-21.
4. Warnke RA, Pulford KAF, Pallesen G, Ralfkiaer E, Brown DC, Gatter KC, et al. (1989) Diagnosis of myelomonocytic and macrophage neoplasms in routinely processed tissue biopsies with monoclonal antibody KP1. Am J Pathol;135:1089-95.