Fibronectin mouse

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Nordic MuBio
Product Code: FI24951-0.1
Product Group: Primary Antibodies
Supplier: Nordic MuBio
CodeSizePrice
FI24951-0.10.1 ml£411.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Radioimmunoassay (RIA)
Storage:
-20°C

Documents

Further Information

Applications Description:
IFA, IHC(P), ELISA, RIA
Background:
This antibody may be used to identify fibronectin during wound healing, during tumour progression, and tumour invasion respectively. Fibronectins are glycoproteins, which occur as dimers of two 200 kDa subunits. They have binding domains for bacterial proteins, collagens, heparin-like molecules and fibrin. Cellular fibronectin is widely distributed in the stroma of malignant tumours. Murine fibronectin 100%, murine collagen type I, III and IV <0.1%, murine laminin <0.1% (RIA at 1:10000 dilution).
Caution:
These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. It should be handled only by trained staff.
Concentration:
app. 1 mg/ml
Immunogen:
Purified fibronectin from murine plasma
Incubation Time:
IHC(P) 60 min at RT or 2-8°C over night
Positive Control:
Mouse skin
Pre-Treatment:
After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg e.g. Art. No. HYA02-50) in TBS 15 min at 37°C. There after non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.
Product:
Affinity purified antibody lyophilized from phosphate buffered solution (non-sterile). Contains antimycotic substance, but no BSA and sodium azide! Reconstitute with 0.5 ml of sterile water and store aliquots at -20°C. For further dilution use appropriate antibody diluent, e.g. Art. No. PU002 for IHC.
Purification Method:
Affinity purified antibody lyophilized from phosphate buffered solution (non-sterile). Contains antimycotic substance, but no BSA and sodium azide! Reconstitute with 0.5 ml of sterile water and store aliquots at -20°C. For further dilution use appropriate antibody diluent, e.g. Art. No. PU002 for IHC.
Secondary Reagents:
Anti-rabbit IgG-conjugates, e.g. anti-rabbit IgG:FITC (Art. No. FI-1000) or anti-rabbit IgG:DyLight488 (Art. No. DI-1488).
Source:
Serum from rabbits immunized with purified mouse fibronectin.
Species Reactivity:
Mouse
Specificity:
Mouse fibronectin
UniProt:
P11276 (FINC_MOUSE)
Working Concentration:
(purified, lyophilized) IFA ? 1:80, IHC(P) ? 1:1000, ELISA ? 1:200 (OD ? 500)

References

1. Andujar MB, Magloire H, Hartmann DJ, Ville G, Grimaud JA (1985) Early mouse molar root development: cellular changes and distribution of fibronectin, laminin and type-IV collagen. Differentiation. 30(2):111-22.
2.Savino W, Itoh T, Imhof BA, Dardenne M (1986) Immunohistochemical studies on the phenotype of murine and human thymic stromal cell lines. Thymus. 8(4):245-56.
3. Villa-Verde DM1, Lagrota-Candido JM, Vannier-Santos MA, Chammas R, Brentani RR, Savino W (1994) Extracellular matrix components of the mouse thymus microenvironment. IV. Modulation of thymic nurse cells by extracellular matrix ligands and receptors. Eur J Immunol. 24(3):659-64.
4. Pankov R and Yamada KM (2002) Fibronectin at a glance. J Cell Sci. Oct 15;115(Pt 20):3861-3
5. Labat-Robert J (2002) Fibronectin in malignancy. Semin Cancer Biol. Jun;12(3):187-95.