Collagen Type I mouse

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Nordic MuBio
Product Code: CO20151-0.1
Product Group: Primary Antibodies
Supplier: Nordic MuBio
CodeSizePrice
CO20151-0.10.1 ml£480.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Chicken (Gallus)
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunoblotting
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Radioimmunoassay (RIA)
  • Western Blot (WB)
Storage:
-20°C

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Further Information

Applications Description:
IHC(P), IFA, ELISA, RIA, IB/WB
Background:
Type I Collagen usually exists as a heterotrimer formed by alpha 1(I) and alpha 2(I) chains and is found in bone, cornea, skin and tendon. In foetal tissues also homotrimers of alpha-1(I) are found, but they are not constituents of normal adult tissues. Collagens consist of a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule consists of a triple-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Mouse collagen type I 100%, mouse collagen type II and IV <0.1%; mouse collage type III <1%; .
Caution:
*These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.
Concentration:
app. 1 mg/ml
Immunogen:
Purified collagen type I from murine skin
Incubation Time:
IHC(P) 60 min at RT or 2-8°C over night
Positive Control:
Mouse skin
Pre-Treatment:
After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg e.g. Art. No. HYA02-50) in TBS 15 min at 37°C. Thereafter non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.
Product:
affinity purified antibody lyophilized from phosphate buffered solution; no BSA and preservative added!
Purification Method:
affinity purified antibody lyophilized from phosphate buffered solution; no BSA and preservative added!
Secondary Reagents:
Anti-rabbit IgG-conjugates, e.g. anti-rabbit IgG:FITC (Art. No. FI-1000) or anti-rabbit IgG:DyLight488 (Art. No. DI-1488).
Species Reactivity:
Mouse; human, chicken and rat collagen type I < 0.1% (RIA at 1:200 dilution)
UniProt:
P11087
Working Concentration:
(purified, lyophilized) IFA ? 1:80, IHC(P) ? 1:500, ELISA ? 1:200 (OD ? 500)

References

1. Demarchez M., Hartmann D.J., Herbage D., Ville G., Prunieras M. (1987) Wound-healing of human-skin transplanted onto the nude-mouse. 2. an immunohistological and ultrastructural study of the epidermal basement-membrane zone reconstruction and connective-t
2. Duprez A., Guerret S., Vignaud J.M., Plenat F., Hartmann D.J., Grimaud J.A. (1987) The interstitial matrix of human carcinomas and sarcomas transplanted to the nude mouse : immunolocalization of some human and murine components. Cell. Mol. Biol. 33, 647-6
3. Andujar M.B., Hartmann D.J., Emonard H., Magloire H. (1988) Distribution and synthesis of type I and type III collagens in developing mouse molar tooth root. Histochemistry 88, 131-140.
4. Plenat F., Vignaud J.M., Guerret-Stocker S., Hartmann D., Duprez K., Duprez A. (1992) Host-Donor interactions in healing of human split-thickness skin grafts onto nude mice: in situ hybridization, immunohistochemical, and histochemical studies. Transplant