Collagen Type IV porcine
| Code | Size | Price |
|---|
| CO20491-0.1 | 0.1 ml | £440.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Porcine (pig)
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunoblotting
- Immunofluorescence (IF)
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Radioimmunoassay (RIA)
- Western Blot (WB)
Storage:
-20°C
Documents
Further Information
Applications Description:
IHC(P), IFA, ELISA, RIA, IB/WB
Background:
Type IV Collagen is a non-fibrillary network of different alpha-chains: alpha 1(IV) to-alpha 6(IV). It is typically found in basal membranes of different organs (e.g. skin, lens, lung, kidney). Collagens consist of a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule consists of a triple-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Porcine collagen type IV 100%, porcine collagens type I, II, and III <0.1%, porcine elastin, fibronectin and laminin < 0.1% (RIA at 1:5000 dilution).
Caution:
*These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.
Concentration:
app. 1 mg/ml
Immunogen:
Purified collagen type IV from porcine kidney tissue
Incubation Time:
IHC(P) 60 min at RT or 2-8°C over night
Positive Control:
Porcine skin or liver
Pre-Treatment:
After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg e.g. Art. No. HYA02-50) in TBS 15 min at 37°C. Thereafter non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.
Product:
Affinity purified antibody lyophilized from phosphate buffered solution; no BSA and preservative added!
Purification Method:
Affinity purified antibody lyophilized from phosphate buffered solution; no BSA and preservative added!
Secondary Reagents:
Anti-rabbit IgG-conjugates, e.g. anti-rabbit IgG:FITC (Art. No. FI-1000) or anti-rabbit IgG:DyLight488 (Art. No. DI-1488).
Source:
Rabbits were immunized with purified collagen type IV from porcine kidney tissue
Species Reactivity:
Pig, cross-reactivity with human < 0.1%, mouse and rat collagen IV <1%
Working Concentration:
(purified, lyophilized) IFA 1:80, IHC(P) 1:500, ELISA >1:200
References
1. Boucard N, Viton C, Agay D, Mari E, Roger T, Chancerelle Y, Domard A ( 2007) The use of physical hydrogels of chitosan for skin regeneration following third-degree burns. Biomaterials. Aug;28(24):3478-88.