Collagen Type I porcine
| Code | Size | Price |
|---|
| CO20191-0.1 | 0.1 ml | £480.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Porcine (pig)
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunoblotting
- Immunofluorescence (IF)
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Radioimmunoassay (RIA)
- Western Blot (WB)
Storage:
-20°C
Documents
Further Information
Applications Description:
IHC(P), IFA, ELISA, RIA, IB/WB
Background:
Type I Collagen usually exists as a heterotrimer formed by alpha 1(I) and alpha 2(I) chains and is found in bone, cornea, skin and tendon. In foetal tissues also homotrimers of alpha-1(I) are found, but they are not constituents of normal adult tissues. Collagens consist of a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule consists of a triple-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Porcine Collagen Type I, cross-reactivity with collagen type III <1%, Collagen type II, IV, V Elastin and Fibronectin <0.1%.
Caution:
*These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.
Concentration:
app. 1 mg/ml
Immunogen:
Purified collagen type I from porcine tendon
Incubation Time:
IHC(P) 60 min at RT or 2-8°C over night
Positive Control:
Porcine skin or tendon
Pre-Treatment:
After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg e.g. Art. No. HYA02-50) in TBS 15 min at 37°C. Thereafter non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.
Product:
affinity purified antibody lyophilized from phosphate buffered solution; no BSA and preservative added!
Purification Method:
affinity purified antibody lyophilized from phosphate buffered solution; no BSA and preservative added!
Secondary Reagents:
Anti-rabbit IgG-conjugates, e.g. anti-rabbit IgG:FITC (Art. No. FI-1000) or anti-rabbit IgG:DyLight488 (Art. No. DI-1488).
Species Reactivity:
Pig, cross-reactivity with rat and cattle <1%, mouse and human <0.1% (RIA at 1:200 dilution)
Working Concentration:
(purified, lyophilized) IFA ? 1:80, IHC(P) ? 1:500, ELISA ? 1:200 (OD ? 500)
References
1. Rousseau C.F, Gagnieu C.H. (2002) In vitro cytocompatibility of porcine type I atelocollagen crosslinked by oxidized glycogen. Biomaterials 23, 1503-1510.
2. Beard HK, Ryvar R, Brown R, Muir H. (1980) Immunochemical localization of collagen types and proteoglycan in pig intervertebral discs. Immunology. Oct;41(2):491-501.
2. Beard HK, Ryvar R, Brown R, Muir H. (1980) Immunochemical localization of collagen types and proteoglycan in pig intervertebral discs. Immunology. Oct;41(2):491-501.