Collagen Type I salmon

IHC(P), IFA, ELISA, RIA

Type I Collagen usually exists as a heterotrimer formed by alpha 1(I) and alpha 2(I) chains and is found in bone, cornea, skin and tendon. In foetal tissues also homotrimers of alpha-1(I) are found, but they are not constituents of normal adult tissues. Collagens consist of a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule consists of a triple-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Salmon (Salmon salar) Collagen type I.

*These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.

app. 1 mg/ml

Purified collagen type I from fish skin

IHC(P) 60 min at RT or 2-8°C over night

Salmon skin or liver

After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg e.g. Art. No. HYA02-50) in TBS 15 min at 37°C. Thereafter non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.

affinity purified antibody lyophilized from phosphate buffered solution; no BSA and preservative added!

affinity purified antibody lyophilized from phosphate buffered solution; no BSA and preservative added!

Anti-rabbit IgG-conjugates, e.g. anti-rabbit IgG:FITC (Art. No. FI-1000) or anti-rabbit IgG:DyLight488 (Art. No. DI-1488).

Salmon, crossreactivity with Sole and Tunafish collagen I <0.4, Goldfish collagen I <0.2, mammalian and avian collagen type I <0.2 (OD ELISA at 1:100 dilution)

C0PUP9 (C0PUP9_SALSA)

(purified, lyophilized) IFA ? 1:40, IHC(P) ? 1:500, ELISA ? 1:200 (OD ? 500)