HAT-tag Antibody [9A2F8]

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ProSci
Product Code: PSI-PM-7663
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-PM-7663-0.02mg0.02mg£150.00
Quantity:
PSI-PM-7663-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG
Antibody Clonality: Monoclonal
Antibody Clone: 9A2F8
Regulatory Status: RUO
Target Species: S-tag
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Shipping:
blue ice
Storage:
HAT-tag antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Images

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Western blot analysis of (1) 200ng, (2) 100 ng, (3) 50 ng, and (4) 25 ng of a HAT-tag-containing recombinant GGP1 protein with HAT-tag antibody at 0.5 μg/ml.
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Western blot analysis of (1) 20 ng of HAT-tagged recombinant GGP1 protein and (2) 20 ng of HAT-tagged recombinant GGP1 protein using 1 μg of HAT-tag antibody to immunoprecipitate and 1 μg/ml anti-GGP1 antibody to detect.
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Western blot analysis of (1) 200ng, (2) 100 ng, (3) 50 ng, and (4) 25 ng of a HAT-tag-containing recombinant GGP1 protein with HAT-tag antibody at 0.5 μg/ml.
Western blot analysis of (1) 20 ng of HAT-tagged recombinant GGP1 protein and (2) 20 ng of HAT-tagged recombinant GGP1 protein using 1 μg of HAT-tag antibody to immunoprecipitate and 1 μg/ml anti-GGP1 antibody to detect.

Documents

Further Information

Additional Names:
Hat-tag, Hat
Application Note:
HAT-tag antibody can be used for detection of HAT-tag epitope-containing recombinant proteins by Western blot at 0.5 - 1 μ/ml.
Background:
Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. HAT-tag is one of the more popular epitope tags for expressed recombinant proteins.
Buffer:
HAT-tag antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
HAT-tag antibody was raised against a synthetic peptide containing KDHLIHNVHKEFHAHAHNK.
NCBI Official Name:
N/A
NCBI Official Symbol:
N/A
NCBI Organism:
Severe acute respiratory syndrome coronavirus 2
Physical State:
Liquid
Purification:
HAT-tag antibody is Protein A chromatography purified.
Research Area:
Other
User NOte:
Optimal dilutions for each application to be determined by the researcher.