Gram's Safranin Solution

Safranin O Solution; Safarin T Solution

Red to very dark red liquid.

477-73-6

32160000

liquid

GHS02

Protect from light and moisture.

H226

InChI=1S/C20H18N4.ClH/c1-12-8-17-19(10-15(12)21)24(14-6-4-3-5-7-14)20-11-16(22)13(2)9-18(20)23-17;/h3-11H,1-2H3,(H3,21,22);1H

OARRHUQTFTUEOS-UHFFFAOYSA-N

Chemical. CAS: 477-73-6. Formula: C20H19ClN4. MW: 350.84. Synthetic. Gram Safranin Solution is a reagent recommended for use in qualitative procedures to differentiate gram-negative from gram-positive organisms. The Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative. The mureine structure of the bacteria walls is the basis of the color affinity. Bacteria will be stained with Gram's crystal violet solution - an aniline dye - in the first step. After the treatment with iod solution, a dye-iod complex will form. During the decolorizing step, this complex stays in the multilayer mureine structures of the Gram-positive bacteria and they will appear blue. Gram-negative bacteria have a monolayer mureine structure only, the dye-iod complex does not stay bound to the cellwall, they will be decolorized. Gram-negative bacteria will be counterstained by safranin solution and appear orange.

MFCD00011759

C20H19ClN4

350.84

Plastic Bottle

III

Gram Safranin Solution is a reagent recommended for use in qualitative procedures to differentiate gram-negative from gram-positive organisms. The Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative. The mureine structure of the bacteria walls is the basis of the color affinity. Bacteria will be stained with Gram's crystal violet solution - an aniline dye - in the first step. After the treatment with iod solution, a dye-iod complex will form. During the decolorizing step, this complex stays in the multilayer mureine structures of the Gram-positive bacteria and they will appear blue. Gram-negative bacteria have a monolayer mureine structure only, the dye-iod complex does not stay bound to the cellwall, they will be decolorized. Gram-negative bacteria will be counterstained by safranin solution and appear orange.

for microscopy

Warning

CC1=C(N)C=C([N+](C2=CC=CC=C2)=C(C=C(N)C(C)=C3)C3=N4)C4=C1.[Cl-]

Soluble in ethanol.

Synthetic.

Non-hazardous

Fluorescent Reagents

41105331

Stable for at least 2 years after receipt when stored at RT.