Gram's Decolorizer Solution
| Code | Size | Price |
|---|
| CDX-G0062-L250 | 250 ml | £35.00 |
Quantity:
| CDX-G0062-LL25 | 2.5 l | £114.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Shipping:
Ambient
Documents
Further Information
Appearance:
Colourless liquid.
CAS:
37348-17-7 (mixture)
Class:
3
EClass:
32160000
Form (Short):
liquid
GHS Symbol:
GHS02,GHS07
Handling Advice:
Protect from light and moisture.
Hazards:
H225, H319, H336
Long Description:
Chemical. CAS: 37348-17-7 (mixture). Formula: . MW: . Synthetic. Gram Decolorizer Solution is a reagent recommended for use in qualitative procedures to differentiate gram-negative from gram-positive organisms. Mixture used to decolorize Gram-negative bacteria. The Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative. The mureine structure of the bacteria walls is the basis of the color affinity. Bacteria will be stained with Gram's crystal violet solution - an aniline dye - in the first step. After the treatment with iod solution, a dye-iod complex will form. During the decolorizing step, this complex stays in the multilayer mureine structures of the Gram-positive bacteria and they will appear blue. Gram-negative bacteria have a monolayer mureine structure only, the dye-iod complex does not stay bound to the cellwall, they will be decolorized. Gram-negative bacteria will be counterstained by safranin solution and appear orange.
MDL:
MFCD00283024
Package Type:
Plastic Bottle
PG:
II
Precautions:
P210, P280, P304+P340+P312, P305+P351+P338, P337+P313, P403+P235
Product Description:
Gram Decolorizer Solution is a reagent recommended for use in qualitative procedures to differentiate gram-negative from gram-positive organisms. Mixture used to decolorize Gram-negative bacteria. The Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative. The mureine structure of the bacteria walls is the basis of the color affinity. Bacteria will be stained with Gram's crystal violet solution - an aniline dye - in the first step. After the treatment with iod solution, a dye-iod complex will form. During the decolorizing step, this complex stays in the multilayer mureine structures of the Gram-positive bacteria and they will appear blue. Gram-negative bacteria have a monolayer mureine structure only, the dye-iod complex does not stay bound to the cellwall, they will be decolorized. Gram-negative bacteria will be counterstained by safranin solution and appear orange.
Purity:
for microscopy
Signal word:
Danger
Source / Host:
Synthetic.
Transportation:
Excepted Quantity
UN Nummer:
UN1993
UNSPSC Category:
Biochemical Reagents
UNSPSC Number:
12352200
Use & Stability:
Stable for at least 2 years after receipt when stored at RT.
References
(1) E. Adams; Stain Technol. 50, 227 (1975) | (2) R. Coico, et al.; Curr. Protoc. Microbiol. Appendix 3-3C (2005)