HPV (Typ 16 u. 18 E6) Human Papilloma Virus Early Protein 6

Nordic MuBio
Product Code: HP305
Product Group: Primary Antibodies
Supplier: Nordic MuBio
CodeSizePrice
HP3053 ml£445.00
Quantity:
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Overview

Host Type: Mouse
Antibody Isotype: IgG
Antibody Clonality: Monoclonal
Antibody Clone: C1P5
Regulatory Status: RUO
Applications:
  • Immunohistochemistry- Frozen Section (IHC-F)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
2-8°C

Further Information

Applications Description:
IHC(C, P)
Background:
This antibody is used for the detection of high risk HPV infections in different tissues. It should always be used when the screening antibody has detected the presence of papillomavirus in suspected tissues. Human Papilloma virus (HPV) is responsible for the development of type and tissue specific papilloma in the oral cavity and in the larynx. In the skin papilloma virus can cause different types of warts and in the ano-genital region it is associated with condyloma or carcinoma. HPV types 1, 6 and 11 usually are associated with benign transformations of the tissues, while e.g. HPV types 16, 18 and 31 seem to be responsible for the development of some carcinoma like cervical carcinoma. The role of HPV in non-melanoic tumours of the skin is under discussion. Human Papillomavirus Early Antigen E6, HPV 16 and 18.
Caution:
*These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.
** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
Immunogen:
HPV type 18 E6 protein produced in bacteria
Incubation Time:
60 min at RT
Positive Control:
HPV-positive vulva carcinoma
Pre-Treatment:
Improvement of staining with unmasking fluid G (Art. No. DE007) or unmasking fluid C (Art. No. DE000)
Product:
Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 30 immunohistochemical tests (100 ?l working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art. No. PU002 if required.
Purification Method:
Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 30 immunohistochemical tests (100 ?l working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art. No. PU002 if required.
Secondary Reagents:
We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005).
Source:
Mouse immunized with recombinant E6 protein fro HPV18
Specificity:
Human Papillomavirus Early Antigen E6 from HPV 16 and 18.
UniProt:
P06463
Working Concentration:
(RTU) neat

References

1. Cowsert et al. (1987) J. Natl. Cancer Inst. 79; 1053-1057.
2. Banks L.J. (1987) J. Gen. Virol. 68; 1351-1355.
3. Cason J., Patel D., Naylor J., et al. (1989) Identification of immunogenic regions of the major coat protein of human papillomavirus type 16 that contain type-restricted epitopes. J. General Virol. 70; 2973-2987.
4. Shepherd P.S., Lunny D., Brooks R., et al. (1992) The detection of human papilloma viruses in cervical biopsies by immunohistochemistry and in situ hybridization. Scand. J. Immunol. 36(11); 69-74.