Mouse anti Episialin
| Code | Size | Price |
|---|
| MUB2060P | 0.1 mg | £326.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: GP1.4
Regulatory Status: RUO
Applications:
- Conjugation
- Flow Cytometry
- Immunoblotting
- Immunocytochemistry (ICC)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Storage:
The antibody is shipped at ambient temperature and may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use an aliquot is thawed slowly in the dark at ambient temperature spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C not refrozen and preferably used the same day. If a slight precipitation occurs upon storage this should be removed by centrifugation. It will not affect the performance or the concentration of the product.
Documents
Further Information
Applications Description:
GP1.4 is suitable for immunoblotting, immunocytochemistry and immunohistochemistry on frozen and paraffin-embedded tissues, and flow cytometry. Optimal antibody dilution should be determined by titration; recommended range is 1:10 ? 1:50 for flow cytometry, and for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:25 ? 1:250 for immunoblotting applications.
Background:
Episialin is also known as MUC1, MAM-6, CA 15-3, PEM and EMA. It is a transmembrane glycoprotein with a large mucin-like extracellular domain that matures through several intermediate forms geneRated by proteolysis, and sequential addition and processing of numerous O-linked glycans that are heavily sialylated. The gene encoding episialin (MUC1) is highly polymorphic and each allele encodes a product that contains a different number of repeats (between 30 and 90) leading to large differences in molecular weight of the protein. Episialin is present in several secretory epithelial cell types.
Caution:
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water.
This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water.
This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
Field of Interest:
Cell Cycle
Label:
FITC
Product:
Each vial contains 500 ul 0.2 mg/ml purified monoclonal antibody in PBS containing 0.05% BSA and 0,05% sodium azide.
Source:
GP1.4 is a Mouse monoclonal IgG1 antibody derived by fusion of Mouse myeloma cells with spleen cells from a Mouse immunized with Human milk fat globule.
Specificity:
GP1.4 reacts with all glycoforms of Episialin
UniProt:
P15941
References
1. Gourevitch, M. M., von Mensdorff-Pouilly, S., Litvinov, S. V., Kenemans, P., van Kamp, G. J., Verstraeten, A. A., and Hilgers, J. (1995). Polymorphic epithelial mucin (MUC-1)-containing circulating immune complexes in carcinoma patients. Br J Cancer 72, 934-938.
2. Norum, L. F., Varaas, T., Kierulf, B., and Nustad, K. (1998). Carcinoma-associated MUC1 detected by immunoradiometric assays. Tumour Biol 19, 134-146.
3. Norum, L. F., Sauren, A. M., Rye, P. D., and Nustad, K. (2001). New immunoassays for MUC1 in breast cancer, Tumour Biol 22, 216-22.
2. Norum, L. F., Varaas, T., Kierulf, B., and Nustad, K. (1998). Carcinoma-associated MUC1 detected by immunoradiometric assays. Tumour Biol 19, 134-146.
3. Norum, L. F., Sauren, A. M., Rye, P. D., and Nustad, K. (2001). New immunoassays for MUC1 in breast cancer, Tumour Biol 22, 216-22.